Evaluation of neurotoxic effects induced by gestational and lactational exposure to contamined eels (Anguilla anguilla) by POP including polychlorinated biphenyl on offspring mice

• Main Author: Dridi, Imen
• Other Authors: Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Université de Lorraine, Rachid Soulimani, Ahmed Landoulsi
• Format: Doctoral or Postdoctoral Thesis
• Language: French
• Published: HAL CCSD 2016
• Subjects: Eels; Gestational and lactational exposure; Motor activity; Memory; Learning; Depressive-Like; Oxidative stress; Acetylcholinesterase activity; Anguilles; Exposition gestationnelle et lactationnelle; Activité motrice; Mémoire; Dépressive like; Stress oxydant; Activité de l’acétylcholinestérase; [SDV.IDA]Life Sciences [q-bio]/Food engineering; [SDV.EE.SANT]Life Sciences [q-bio]/Ecology; environment/Health; Anguilla anguilla
• Online Access: https://theses.hal.science/tel-01537678; https://theses.hal.science/tel-01537678/document; https://theses.hal.science/tel-01537678/file/DDOC_T_2016_0269_DRIDI.pdf

The objective of the present study is the evaluation of gestational and lactational exposure to contaminated eels, containing Σ6 NDL-PCBs, on learning, memory, motor activity, resignation behavior, oxidative status and acetylcholinesterase activity of the offspring mice. For this purpose female mice were orally exposed during gestational and breast feeding period (from JG6 to JPN 21), through standard diet containing contaminated eel with Σ6 NDL-PCBs or no. Three doses of Σ 6 NDL-PCBs were chosen: 85ng / kg / day, 216 ng / kg / day and 400ng / kg / day. A control group of female mice, receiving the standard diet without fish, was used. Results showed that gestational and lactational exposure to contaminated eels, containing Σ6 NDL-PCBs, did not affect the memory and learning capacities. However the early-life exposure to polluted eels containing Σ6 NDL-PCBs caused late, persistent and gender dependent neurobehavioral hyperactive effects in offspring mice that affects only offspring females. Furthermore our results suggest that the consumption of polluted fish by pregnant and lactating dams might cause neurobehavioral depression only in their male offspring, at progressive life stage. Moreover the study of oxidative status on the three brain areas (prefrontal cortex, hippocampus and cerebellum), revealed that the treatment had affected the activity of antioxidant enzymes (Cat, SOD) only in the prefrontal cortex, hippocampus of male mice. The consequences of oxidative stress in this study were revealed by an increase of protein carbonyl levels in the hippocampus and the prefrontal cortex of offspring male mice, exposed to chemically contaminated fish containing Σ6 NDL-PCBs at the dose of 85 and 216 ng / kg / day, respectively. However the treatment had no effect on the lipids. Indeed there were no significant differences in the MDA rate, in the three studied regions of the brain, between treated and control mice. Moreover, gestational and lactational exposure to contaminated eels, containing Σ6NDL-PCB at the dose ...