Characterization of polymorphic microsatellite DNA markers in the black-tailed godwit (Limosa limosa:Aves)
We isolated and tested 16 microsatellite loci in black-tailed godwits from the Netherlands (Limosa limosa limosa), and from Australasia (subspecies melanuroides). One locus was monomorphic, two loci had null-alleles and one was significantly heterozygote deficient. The remaining 12 polymorphic loci...
Published in: | Molecular Ecology Resources |
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Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2009
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Subjects: | |
Online Access: | https://hdl.handle.net/11370/f76a1120-051c-49bf-b860-7bcb79c0f95e https://research.rug.nl/en/publications/f76a1120-051c-49bf-b860-7bcb79c0f95e https://doi.org/10.1111/j.1755-0998.2009.02685.x https://pure.rug.nl/ws/files/6735409/2009MolEcolResourVerkuil.pdf |
Summary: | We isolated and tested 16 microsatellite loci in black-tailed godwits from the Netherlands (Limosa limosa limosa), and from Australasia (subspecies melanuroides). One locus was monomorphic, two loci had null-alleles and one was significantly heterozygote deficient. The remaining 12 polymorphic loci had on average 7.9 alleles (range 5-11) and the mean expected heterozygosity was 0.69. No significant linkage disequilibrium between the loci was observed and all loci were autosomal. Fourteen loci were successfully cross-amplified in bar-tailed godwit (Limosa lapponica). |
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