Characterization of polymorphic microsatellite DNA markers in the black-tailed godwit (Limosa limosa:Aves)

We isolated and tested 16 microsatellite loci in black-tailed godwits from the Netherlands (Limosa limosa limosa), and from Australasia (subspecies melanuroides). One locus was monomorphic, two loci had null-alleles and one was significantly heterozygote deficient. The remaining 12 polymorphic loci...

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Bibliographic Details
Published in:Molecular Ecology Resources
Main Authors: Verkuil, Yvonne I., Trimbos, Krijn, Haddrath, Oliver, Baker, Allan J., Piersma, Theunis
Format: Article in Journal/Newspaper
Language:English
Published: 2009
Subjects:
black-tailed godwit
ground-nesting shorebird
Limosa limosa
microsatellite
population genetics
SOFTWARE
Online Access:https://hdl.handle.net/11370/f76a1120-051c-49bf-b860-7bcb79c0f95e
https://research.rug.nl/en/publications/f76a1120-051c-49bf-b860-7bcb79c0f95e
https://doi.org/10.1111/j.1755-0998.2009.02685.x
https://pure.rug.nl/ws/files/6735409/2009MolEcolResourVerkuil.pdf
Description
Summary:We isolated and tested 16 microsatellite loci in black-tailed godwits from the Netherlands (Limosa limosa limosa), and from Australasia (subspecies melanuroides). One locus was monomorphic, two loci had null-alleles and one was significantly heterozygote deficient. The remaining 12 polymorphic loci had on average 7.9 alleles (range 5-11) and the mean expected heterozygosity was 0.69. No significant linkage disequilibrium between the loci was observed and all loci were autosomal. Fourteen loci were successfully cross-amplified in bar-tailed godwit (Limosa lapponica).

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