EVOLUTIONARY RELATIONSHIPS BETWEEN 4 SPECIES OF CLADOPHORA (CLADOPHORALES, CHLOROPHYTA) BASED ON DNA-DNA HYBRIDIZATION
Analysis of the reassociation kinetics of the DNA from Cladophora pellucida (Huds.) Kutz. indicates that the genome of this benthic alga is comprised of approximately 75% repetitive sequences. Single-copy sequences reassociated with a rate constant of 1.8 x 10(-3) M-1.s-1, which corresponds to a hap...
Main Authors: | , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
1991
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Subjects: | |
Online Access: | http://hdl.handle.net/11370/f3b5db7d-e639-4ea9-a179-7c2f5a7c292b https://research.rug.nl/en/publications/evolutionary-relationships-between-4-species-of-cladophora-cladophorales-chlorophyta-based-on-dnadna-hybridization(f3b5db7d-e639-4ea9-a179-7c2f5a7c292b).html |
Summary: | Analysis of the reassociation kinetics of the DNA from Cladophora pellucida (Huds.) Kutz. indicates that the genome of this benthic alga is comprised of approximately 75% repetitive sequences. Single-copy sequences reassociated with a rate constant of 1.8 x 10(-3) M-1.s-1, which corresponds to a haploid genome size of 4.7 x 10(8) bp. Genotypic relationships between members of the form section Longiarticulatae were determined by the method of DNA-DNA hybridization. No significant divergence was observed between the single-copy sequences of C. pellucida isolates from the East Atlantic coast and Mediterranean Sea. Cladophora feredayi Harv. and C. att. ad pellucida from Australia and C. pellucidoidea van den Hoek from the West Atlantic coast were highly and about equally divergent from C. pellucida. The data support the hypothesis that the West Atlantic-West Pacific divergence reflects the middle Miocene closure of the Mediterranean-Indo-Pacific seaways, and the hypothesis that the Northwest Atlantic-Northeast Atlantic divergence reflects the middle Miocene thermal separation of these coasts. |
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