Lack of circadian patterns in vasoactive intestinal polypeptide release and variability in vasopressin release in vole suprachiasmatic nuclei in vitro

Organotypic hypothalamic cultures of neonatal rats comprising the suprachiasmatic nuclei (SCN) produce stable 20 h release patterns of vasoactive intestinal polypeptide (VIP) and arginine-vasopressin (AVP). Compared with rats, voles show variably expressed circadian activity patterns. In this study...

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Bibliographic Details
Published in:Neuroscience Letters
Main Authors: Gerkema, M P, Shinohara, K, Kimura, F
Format: Article in Journal/Newspaper
Language:English
Published: 1999
Subjects:
circadian
ultradian
vasopressin
vasoactive intestinal polypeptide
vole
suprachiasmatic nucleus
organotypic
ARGININE-VASOPRESSIN
EFFERENT PROJECTIONS
MICROTUS-ARVALIS
RAT
RHYTHMICITY
Common vole
Microtus arvalis
Online Access:https://hdl.handle.net/11370/eb0c43f1-d6e5-403b-8ffb-aa2af84bcd81
https://research.rug.nl/en/publications/eb0c43f1-d6e5-403b-8ffb-aa2af84bcd81
https://doi.org/10.1016/S0304-3940(98)00919-7
Description
Summary:Organotypic hypothalamic cultures of neonatal rats comprising the suprachiasmatic nuclei (SCN) produce stable 20 h release patterns of vasoactive intestinal polypeptide (VIP) and arginine-vasopressin (AVP). Compared with rats, voles show variably expressed circadian activity patterns. In this study we measured neuropeptidergic release patterns in organotypic SCN cultures of neonatal common voles (Microtus arvalis, n = 6). Slices were prepared at postnatal day 6. After 14 days of incubation, 2 h samples of medium were collected during 50 h. None of the vole SCN slices showed a circadian modulation in VIP release, Peaks in AVP occurred, 20 h apart from each other, in four of six vole SCN slices. These findings contrast with the concurrent release patterns of VIP and AVP in rat SCN slices. The results suggest an independent role of both neuropeptides in the oscillatory output pathways of the circadian pacemaker in the common vole. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

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