UV-B induces DNA damage and DNA synthesis delay in the marine diatom Cyclotella sp.

The effect of UV-B on the occurrence of DNA damage and consequences for the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dose (biologically effe...

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Bibliographic Details
Main Authors: Buma, A.G.J., van Hannen, E.J, Veldhuis, M.J W, Gieskes, W.W C
Format: Article in Journal/Newspaper
Language:English
Published: 1996
Subjects:
cell cycle
DNA damage
flow-cytometry
marine diatom
thymine dimers
UV-B
ULTRAVIOLET-RADIATION
ANTARCTICA
DOSIMETER
DIMERS
OCEAN
CELLS
Antarc*
Antarctica
Online Access:https://hdl.handle.net/11370/d8389324-a9b3-4977-a60e-52078d777078
https://research.rug.nl/en/publications/d8389324-a9b3-4977-a60e-52078d777078
Description
Summary:The effect of UV-B on the occurrence of DNA damage and consequences for the cell cycle were studied in the marine diatom Cyclotella sp. DNA damage was quantified by immunofluorescent detection of thymine dimers in nuclear DNA of single cells using flow cytometry. A total UV-B dose (biologically effective dose BE(DNA300nm)) of 1.05 kJ m(-2) caused detectable damage in about 20% of the exposed population. In the period after the UV-B treatment, when the culture was exposed to PAR (450 mu mol m(-2)s(-1)) only, thymine dimers were removed; after 8 hours none of these photoproducts remained. Cellular DNA content measurements and quantification of the fraction of recently divided cells revealed that the DNA synthesis rate as well as the rate of cell division were reduced during and shortly after UV-B exposure. Apparently, UV-B irradiation extends the cell cycle of Cyclotella sp. in the S (DNA synthesis) phase until the dimers are removed.

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