New primers for molecular sex identification of waders

Determining the sex of individuals through molecular methods is an increasingly important research tool, particularly for studies of sexually monomorphic species. However, currently available primer sets to molecularly sex birds are known to cause difficulties in sexing Red Knots Calidris canutus an...

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Bibliographic Details
Published in:Wader Study
Main Authors: van der Velde, Marco, Haddrath, Oliver, Verkuil, Yvonne I., Baker, Allan J., Piersma, Theun
Format: Article in Journal/Newspaper
Language:English
Published: 2017
Subjects:
shorebirds
molecular sexing
Red Knot
primers 2602F & 2669R
CHD gene
Calidris canutus
Online Access:https://hdl.handle.net/11370/ac23267e-4c42-42e3-866a-170ab610fc71
https://research.rug.nl/en/publications/ac23267e-4c42-42e3-866a-170ab610fc71
https://doi.org/10.18194/ws.00069
https://pure.rug.nl/ws/files/134418964/New_primers_for_molecular_sex_identification_of_waders.pdf
Description
Summary:Determining the sex of individuals through molecular methods is an increasingly important research tool, particularly for studies of sexually monomorphic species. However, currently available primer sets to molecularly sex birds are known to cause difficulties in sexing Red Knots Calidris canutus and several other waders, due to amplification failure of, and polymorphisms on, the CHD-Z gene. To counter these problems, we developed a new primer set, 2602F/2669R, and show that these primers can be used to reliably sex Red Knots and eight other wader species in four genera. We expect these primers to be useful in a wide range of wader and possibly non-wader species.

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