Evolutionary applications of MIRs and SINEs

It is believed that short interspersed elements (SINEs) are irreversibly inserted into genomes. We use this concept to try to deduce the evolution of whales using sequence and hybridization studies. The observation that microsatellites are associated with SINEs lead us to screen sequences surroundin...

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Bibliographic Details
Published in:Animal Genetics
Main Authors: Buchanan, F, Crawford, A, Strobeck, C, Palsboll, P, Plante, Y
Format: Article in Journal/Newspaper
Language:English
Published: 1999
Subjects:
artiodactyl
cetacean
mammalian-wide interspersed repeat
short interspersed element
MOLECULAR EVIDENCE
MICROSATELLITES
RETROPOSONS
CETACEANS
Beluga
Beluga*
Online Access:https://hdl.handle.net/11370/ab0f8aaa-d7ee-4322-b70e-5573e3572c13
https://research.rug.nl/en/publications/ab0f8aaa-d7ee-4322-b70e-5573e3572c13
https://doi.org/10.1046/j.1365-2052.1999.00388.x
Description
Summary:It is believed that short interspersed elements (SINEs) are irreversibly inserted into genomes. We use this concept to try to deduce the evolution of whales using sequence and hybridization studies. The observation that microsatellites are associated with SINEs lead us to screen sequences surrounding cetacean microsatellites for artiodactyl-derived SINEs. Two sequences that were thought to be cetacean SINEs and the bovine SINE were aligned for comparison to sequences flanking microsatellites from ungulates. The bovine SINE was observed only in ruminants while CetSINE1 and 2 were found in mammals. Hybridization studies using these three SINEs revealed that CetSINE1 was found in all ungulates and cetaceans with the strongest hybridization signal observed in the hippopotamus and beluga; CetSINE2 hybridized to all ungulate suborders, while the bovine SINE was only observed in Ruminantia. It is proposed that these putative SINEs are not 'generic' SINEs but mammalian-wide interspersed repeats (MIRs). Caution is urged: what initially appears to be a SINE may instead be a MIR and have reduced evolutionary resolving power.

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