Diversity of ribulose-1,5-bisphosphate carboxylase/oxygenase large-subunit genes in the MgCl2-dominated deep hypersaline anoxic basin discovery

Partial sequences of the form I (cbbL) and form II (cbbM) of the ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) large subunit genes were obtained from the brine and interface of the MgCl2-dominated deep hypersaline anoxic basin Discovery. CbbL and cbbM genes were found in both brine and i...

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Bibliographic Details
Published in:FEMS Microbiology Letters
Main Author: van der Wielen, Paul W. J. J.
Format: Article in Journal/Newspaper
Language:English
Published: 2006
Subjects:
RuBisCO
phylogenetic diversity
deep hypersaline anoxic basin
magnesium chloride
chemoautotrophy
RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE
MEDITERRANEAN SEA
SPECIES VARIATION
RED-SEA
OXYGENASE
MICROORGANISMS
EVOLUTION
ANTARCTICA
GRADIENT
Antarc*
Antarctica
Online Access:https://hdl.handle.net/11370/9fc9e666-6c15-4358-a29b-94c30728eda1
https://research.rug.nl/en/publications/9fc9e666-6c15-4358-a29b-94c30728eda1
https://doi.org/10.1111/j.1574-6968.2006.00284.x
Description
Summary:Partial sequences of the form I (cbbL) and form II (cbbM) of the ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) large subunit genes were obtained from the brine and interface of the MgCl2-dominated deep hypersaline anoxic basin Discovery. CbbL and cbbM genes were found in both brine and interface of the Discovery Basin but were absent in the overlying seawater. The diversity of both genes in the brine and interface was low, which might caused by the extreme saline conditions in Discovery of similar to 5 M MgCl2. None of the retrieved sequences were closely related to sequences deposited in the GenBank database. A phylogenetic analysis demonstrated that the cbbL sequences were affiliated with a Thiobacillus sp. or with one of the RuBisCO genes from Hydrogenovibrio marinus. The cbbM sequences clustered with thiobacilli or formed a new group with no close relatives. The results implicate that bacteria with the potential for carbon dioxide fixation and chemoautotrophy are present in the Discovery Basin. This is the first report demonstrating that RuBisCO genes are present under hypersaline conditions of 5 M MgCl2.

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