Archaeal amoA gene diversity points to distinct biogeography of ammonia-oxidizing Crenarchaeota in the ocean

Mesophilic ammonia-oxidizing Archaea (AOA) are abundant in a diverse range of marine environments, including the deep ocean, as revealed by the quantification of the archaeal amoA gene encoding the alpha-subunit of the ammonia monooxygenase. Using two different amoA primer sets, two distinct ecotype...

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Bibliographic Details
Published in:Environmental Microbiology
Main Authors: Sintes, Eva, Bergauer, Kristin, De Corte, Daniele, Yokokawa, Taichi, Herndl, Gerhard J.
Format: Article in Journal/Newspaper
Language:English
Published: 2013
Subjects:
PACIFIC SUBTROPICAL GYRE
NEIGHBOR-JOINING METHOD
NITRIFYING ARCHAEA
ATLANTIC-OCEAN
NORTH-ATLANTIC
MARINE CRENARCHAEOTA
MESOPELAGIC OCEAN
MEDITERRANEAN SEA
AMINO-ACIDS
BACTERIA
Arctic
Pacific
North Atlantic
Online Access:https://hdl.handle.net/11370/8b420713-75f9-472e-8856-c3e724d7bcd1
https://research.rug.nl/en/publications/8b420713-75f9-472e-8856-c3e724d7bcd1
https://doi.org/10.1111/j.1462-2920.2012.02801.x
https://pure.rug.nl/ws/files/132283117/Archaeal_amoA_gene_diversity_points_to_distinct_biogeography_of_ammonia_oxidizing_Crenarchaeota.pdf
Description
Summary:Mesophilic ammonia-oxidizing Archaea (AOA) are abundant in a diverse range of marine environments, including the deep ocean, as revealed by the quantification of the archaeal amoA gene encoding the alpha-subunit of the ammonia monooxygenase. Using two different amoA primer sets, two distinct ecotypes of marine Crenarchaeota Group I (MCGI) were detected in the waters of the tropical Atlantic and the coastal Arctic. The HAC-AOA ecotype (high ammonia concentration AOA) was approximate to 8000 times and 15 times more abundant in the coastal Arctic and the top 300m layer of the open equatorial Atlantic, respectively, than the LAC-AOA (low ammonia concentration AOA) ecotype. In contrast, the LAC-AOA ecotype dominated the lower meso- and bathypelagic waters of the tropical Atlantic (approximate to 50 times more abundant than the HAC-AOA) where ammonia concentrations are well below the detection limit using conventional spectrophotometric or fluorometric methods. Cluster analysis of the sequences from the clone libraries obtained by the two amoA primer sets revealed two phylogenetically distinct clusters. Taken together, our results suggest the presence of two ecotypes of archaeal ammonia oxidizers corresponding to the medium (1.24 mu M on average in the coastal Arctic) and low ammonia concentration (

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