In situ impact of solar ultraviolet radiation on photosynthesis and DNA in temperate marine phytoplankton
In situ experiments were conducted at various depths in the water column to determine the impact of solar UV radiation (280 to 400 nm) upon photosynthesis and DNA of natural phytoplankton assemblages from mid-latitudes of Patagonia (Bahia Bustamante, Chubut, Argentina; 45 degreesS, 66.5 degreesW). T...
Published in: | Marine Ecology Progress Series |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2001
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Online Access: | https://hdl.handle.net/11370/81d7f86f-ae88-4960-baef-b92028050e36 https://research.rug.nl/en/publications/81d7f86f-ae88-4960-baef-b92028050e36 https://doi.org/10.3354/meps211043 https://pure.rug.nl/ws/files/62523860/In_situ_impact_of_solar_ultraviolet_radiation_on_photosynthesis.pdf |
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ftunigroningenpu:oai:pure.rug.nl:publications/81d7f86f-ae88-4960-baef-b92028050e36 2024-06-23T07:47:47+00:00 In situ impact of solar ultraviolet radiation on photosynthesis and DNA in temperate marine phytoplankton Helbling, E.W Buma, A.G.J. de Boer, M.K. Villafane, V 2001-02-14 application/pdf https://hdl.handle.net/11370/81d7f86f-ae88-4960-baef-b92028050e36 https://research.rug.nl/en/publications/81d7f86f-ae88-4960-baef-b92028050e36 https://doi.org/10.3354/meps211043 https://pure.rug.nl/ws/files/62523860/In_situ_impact_of_solar_ultraviolet_radiation_on_photosynthesis.pdf eng eng https://research.rug.nl/en/publications/81d7f86f-ae88-4960-baef-b92028050e36 info:eu-repo/semantics/openAccess Helbling , E W , Buma , A G J , de Boer , M K & Villafane , V 2001 , ' In situ impact of solar ultraviolet radiation on photosynthesis and DNA in temperate marine phytoplankton ' , Marine Ecology Progress Series , vol. 211 , pp. 43-49 . https://doi.org/10.3354/meps211043 UV phytoplankton DNA CPD thymine dimers photosynthesis inhibition mid-latitudes EFFECTIVE UV-RADIATION OZONE DEPLETION ANTARCTIC PHYTOPLANKTON B RADIATION WATERS INHIBITION GROWTH OCEAN article 2001 ftunigroningenpu https://doi.org/10.3354/meps211043 2024-06-03T16:02:45Z In situ experiments were conducted at various depths in the water column to determine the impact of solar UV radiation (280 to 400 nm) upon photosynthesis and DNA of natural phytoplankton assemblages from mid-latitudes of Patagonia (Bahia Bustamante, Chubut, Argentina; 45 degreesS, 66.5 degreesW). The effects of UV radiation were significant at the surface; however, the impact decreased rapidly with depth: at 3 m there was no measurable DNA damage accumulation, whereas at 6 m photosynthetic inhibition was almost zero. UV-A radiation (315 to 400 nm) was mostly responsible for photosynthetic inhibition, while UV-B radiation (280 to 315 nm) had a lesser effect on this process. However, UV-B radiation was very effective in damaging the DNA through the formation of cyclobutane pyrimidine dimers (CPDs) in surface waters. The high initial CPD level found in the natural phytoplankton assemblage decreased when samples were incubated at 3 or 6 m, indicating that at these depths repair, dilution or disappearance of damage occurred. Phytoplankton assemblages were dominated by cells less than 2 mum in effective diameter; this cell size category seems to be more resistant to photosynthetic inhibition, but vulnerable to CPD accumulation, as compared with larger eukaryotic phytoplankters (i.e., Phaeodactylum sp.). Article in Journal/Newspaper Antarc* Antarctic University of Groningen research database Antarctic Argentina Chubut ENVELOPE(-62.533,-62.533,-76.100,-76.100) Patagonia Patagonia bahia ENVELOPE(-63.187,-63.187,-64.458,-64.458) Marine Ecology Progress Series 211 43 49 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
UV phytoplankton DNA CPD thymine dimers photosynthesis inhibition mid-latitudes EFFECTIVE UV-RADIATION OZONE DEPLETION ANTARCTIC PHYTOPLANKTON B RADIATION WATERS INHIBITION GROWTH OCEAN |
spellingShingle |
UV phytoplankton DNA CPD thymine dimers photosynthesis inhibition mid-latitudes EFFECTIVE UV-RADIATION OZONE DEPLETION ANTARCTIC PHYTOPLANKTON B RADIATION WATERS INHIBITION GROWTH OCEAN Helbling, E.W Buma, A.G.J. de Boer, M.K. Villafane, V In situ impact of solar ultraviolet radiation on photosynthesis and DNA in temperate marine phytoplankton |
topic_facet |
UV phytoplankton DNA CPD thymine dimers photosynthesis inhibition mid-latitudes EFFECTIVE UV-RADIATION OZONE DEPLETION ANTARCTIC PHYTOPLANKTON B RADIATION WATERS INHIBITION GROWTH OCEAN |
description |
In situ experiments were conducted at various depths in the water column to determine the impact of solar UV radiation (280 to 400 nm) upon photosynthesis and DNA of natural phytoplankton assemblages from mid-latitudes of Patagonia (Bahia Bustamante, Chubut, Argentina; 45 degreesS, 66.5 degreesW). The effects of UV radiation were significant at the surface; however, the impact decreased rapidly with depth: at 3 m there was no measurable DNA damage accumulation, whereas at 6 m photosynthetic inhibition was almost zero. UV-A radiation (315 to 400 nm) was mostly responsible for photosynthetic inhibition, while UV-B radiation (280 to 315 nm) had a lesser effect on this process. However, UV-B radiation was very effective in damaging the DNA through the formation of cyclobutane pyrimidine dimers (CPDs) in surface waters. The high initial CPD level found in the natural phytoplankton assemblage decreased when samples were incubated at 3 or 6 m, indicating that at these depths repair, dilution or disappearance of damage occurred. Phytoplankton assemblages were dominated by cells less than 2 mum in effective diameter; this cell size category seems to be more resistant to photosynthetic inhibition, but vulnerable to CPD accumulation, as compared with larger eukaryotic phytoplankters (i.e., Phaeodactylum sp.). |
format |
Article in Journal/Newspaper |
author |
Helbling, E.W Buma, A.G.J. de Boer, M.K. Villafane, V |
author_facet |
Helbling, E.W Buma, A.G.J. de Boer, M.K. Villafane, V |
author_sort |
Helbling, E.W |
title |
In situ impact of solar ultraviolet radiation on photosynthesis and DNA in temperate marine phytoplankton |
title_short |
In situ impact of solar ultraviolet radiation on photosynthesis and DNA in temperate marine phytoplankton |
title_full |
In situ impact of solar ultraviolet radiation on photosynthesis and DNA in temperate marine phytoplankton |
title_fullStr |
In situ impact of solar ultraviolet radiation on photosynthesis and DNA in temperate marine phytoplankton |
title_full_unstemmed |
In situ impact of solar ultraviolet radiation on photosynthesis and DNA in temperate marine phytoplankton |
title_sort |
in situ impact of solar ultraviolet radiation on photosynthesis and dna in temperate marine phytoplankton |
publishDate |
2001 |
url |
https://hdl.handle.net/11370/81d7f86f-ae88-4960-baef-b92028050e36 https://research.rug.nl/en/publications/81d7f86f-ae88-4960-baef-b92028050e36 https://doi.org/10.3354/meps211043 https://pure.rug.nl/ws/files/62523860/In_situ_impact_of_solar_ultraviolet_radiation_on_photosynthesis.pdf |
long_lat |
ENVELOPE(-62.533,-62.533,-76.100,-76.100) ENVELOPE(-63.187,-63.187,-64.458,-64.458) |
geographic |
Antarctic Argentina Chubut Patagonia Patagonia bahia |
geographic_facet |
Antarctic Argentina Chubut Patagonia Patagonia bahia |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Helbling , E W , Buma , A G J , de Boer , M K & Villafane , V 2001 , ' In situ impact of solar ultraviolet radiation on photosynthesis and DNA in temperate marine phytoplankton ' , Marine Ecology Progress Series , vol. 211 , pp. 43-49 . https://doi.org/10.3354/meps211043 |
op_relation |
https://research.rug.nl/en/publications/81d7f86f-ae88-4960-baef-b92028050e36 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.3354/meps211043 |
container_title |
Marine Ecology Progress Series |
container_volume |
211 |
container_start_page |
43 |
op_container_end_page |
49 |
_version_ |
1802637956025942016 |