In situ impact of solar ultraviolet radiation on photosynthesis and DNA in temperate marine phytoplankton

In situ experiments were conducted at various depths in the water column to determine the impact of solar UV radiation (280 to 400 nm) upon photosynthesis and DNA of natural phytoplankton assemblages from mid-latitudes of Patagonia (Bahia Bustamante, Chubut, Argentina; 45 degreesS, 66.5 degreesW). T...

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Bibliographic Details
Published in:Marine Ecology Progress Series
Main Authors: Helbling, E.W, Buma, A.G.J., de Boer, M.K., Villafane, V
Format: Article in Journal/Newspaper
Language:English
Published: 2001
Subjects:
UV
phytoplankton
DNA
CPD
thymine dimers
photosynthesis inhibition
mid-latitudes
EFFECTIVE UV-RADIATION
OZONE DEPLETION
ANTARCTIC PHYTOPLANKTON
B RADIATION
WATERS
INHIBITION
GROWTH
OCEAN
Antarctic
Argentina
Chubut
Patagonia
Patagonia bahia
Antarc*
Online Access:https://hdl.handle.net/11370/81d7f86f-ae88-4960-baef-b92028050e36
https://research.rug.nl/en/publications/81d7f86f-ae88-4960-baef-b92028050e36
https://doi.org/10.3354/meps211043
https://pure.rug.nl/ws/files/62523860/In_situ_impact_of_solar_ultraviolet_radiation_on_photosynthesis.pdf
Description
Summary:In situ experiments were conducted at various depths in the water column to determine the impact of solar UV radiation (280 to 400 nm) upon photosynthesis and DNA of natural phytoplankton assemblages from mid-latitudes of Patagonia (Bahia Bustamante, Chubut, Argentina; 45 degreesS, 66.5 degreesW). The effects of UV radiation were significant at the surface; however, the impact decreased rapidly with depth: at 3 m there was no measurable DNA damage accumulation, whereas at 6 m photosynthetic inhibition was almost zero. UV-A radiation (315 to 400 nm) was mostly responsible for photosynthetic inhibition, while UV-B radiation (280 to 315 nm) had a lesser effect on this process. However, UV-B radiation was very effective in damaging the DNA through the formation of cyclobutane pyrimidine dimers (CPDs) in surface waters. The high initial CPD level found in the natural phytoplankton assemblage decreased when samples were incubated at 3 or 6 m, indicating that at these depths repair, dilution or disappearance of damage occurred. Phytoplankton assemblages were dominated by cells less than 2 mum in effective diameter; this cell size category seems to be more resistant to photosynthetic inhibition, but vulnerable to CPD accumulation, as compared with larger eukaryotic phytoplankters (i.e., Phaeodactylum sp.).

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