Effect of Candida antarctica Lipase B Immobilization on the Porous Structure of the Carrier

A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a sig...

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Bibliographic Details
Published in:Macromolecular Bioscience
Main Authors: Miletic, Nemanja, Vukovic, Zorica, Nastasovic, Aleksandra, Loos, Katja, Miletić, Nemanja, Vuković, Zorica, Nastasović, Aleksandra
Format: Article in Journal/Newspaper
Language:English
Published: 2011
Subjects:
diffusion
enzymes
immobilization
lipase
macroporous copolymers
GLYCOL DIMETHACRYLATE) RESINS
GLYCIDYL METHACRYLATE
REACTIVE POLYMERS
ORGANIC-SOLVENTS
PORE-SIZE
COPOLYMERS
ETHYLENEDIMETHACRYLATE
CHROMATOGRAPHY
INVERTASE
Antarc*
Antarctica
Online Access:https://hdl.handle.net/11370/6f9fcb7b-5de9-49e9-afdb-b0623437cc1d
https://research.rug.nl/en/publications/6f9fcb7b-5de9-49e9-afdb-b0623437cc1d
https://doi.org/10.1002/mabi.201100127
https://pure.rug.nl/ws/files/6771198/2011MacromolBiosciMiletic.pdf
Description
Summary:A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1-fold), the specific surface area (up to 3.2-fold) and the specific volume (up to 2.1-fold) of the epoxy resin. A similar behaviour is observed for glutaraldehyde-modified epoxy resins. The influences of the resin porosity properties on the loading of Candida antarctica lipase B during immobilization and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized lipase are studied.

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