Ultraviolet-B-induced cyclobutane-pyrimidine dimer formation and repair in arctic marine macrophytes

The significance of ultraviolet-B radiation (UVBR: 280-315 nm)-induced DNA damage as a stress factor for Arctic marine macrophytes was examined in the Kongsfjord (Spitsbergen, 78degrees55.5'N, 11degrees56.0'E) in summer. UVBR penetration in the water column was monitored as accumulation of...

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Bibliographic Details
Main Authors: van de Poll, W.H., Hanelt, D, Hoyer, K., Buma, A.G.J., Breeman, Arno
Format: Article in Journal/Newspaper
Language:English
Published: 2002
Subjects:
INDUCED DNA-DAMAGE
PHOTOSYNTHETICALLY ACTIVE RADIATION
UV-RADIATION
SOLAR-RADIATION
OZONE DEPLETION
EXCISION-REPAIR
AMINO-ACIDS
GROWTH-RATE
ARABIDOPSIS
PHOTOLYASE
Arctic
Kongsfjord
Kongsfjord*
Spitsbergen
Online Access:https://hdl.handle.net/11370/4e7c878d-9d57-4cf9-85c3-ae812cb6f191
https://research.rug.nl/en/publications/4e7c878d-9d57-4cf9-85c3-ae812cb6f191
Description
Summary:The significance of ultraviolet-B radiation (UVBR: 280-315 nm)-induced DNA damage as a stress factor for Arctic marine macrophytes was examined in the Kongsfjord (Spitsbergen, 78degrees55.5'N, 11degrees56.0'E) in summer. UVBR penetration in the water column was monitored as accumulation of cyclobutane-pyrimidine dimers (CPD) in bare DNA. This showed that UVBR transparency of the fjord was variable, with 1% depths ranging between 4 and 8 m. In addition, induction and repair kinetics of CPD were studied in several subtidal macrophytes obtained from the Kongsfjord (5-15 m). Surface exposure experiments demonstrated CPD accumulation in Palmaria palmata, Devaleraea ramentacea, Phycodrys rubens, Coccotylus truncates and Odonthalia dentata. In artificial light, field collected material of P. palmata, D. ramentacea, P. rubens and Laminaria saccharina showed efficient CPD repair, with only 10% of the artificially induced CPD remaining after 5 h. No significant differences in repair rate were observed among these species. CPD repair was slower or absent in O. dentata, C. truncates and Monostroma arcticum, indicating that fast repair mechanisms such as photolyase were not continuously expressed in these species. CPD repair rates were not directly related to the vertical distribution of algae in the water column and to the reported UV sensitivity of the examined species. Dosimeter incubations showed that maximal exposure to DNA damaging wavelengths was low for all examined species. Furthermore, most species collected below the 1% depth for DNA damage displayed efficient CPD repair, suggesting that UVBR-induced CPD currently impose a minor threat for mature stages of these species growing in the Kongsfjord, Spitsbergen.

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