Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B
PANGMA nanofibers and nanomats with fiber diameters of 200-300 nanometers were fabricated by electrospinning. Cal-B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal-B-immobilized PANGMA nanomats were assayed and compared with th...
Published in: | Macromolecular Chemistry and Physics |
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Main Authors: | , , , , , |
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Language: | English |
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2011
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Online Access: | https://hdl.handle.net/11370/3f230449-e252-4108-a5ca-4d86bd6fd85d https://research.rug.nl/en/publications/3f230449-e252-4108-a5ca-4d86bd6fd85d https://doi.org/10.1002/macp.201000536 https://pure.rug.nl/ws/files/6759274/2011MacromolChemPhysDai.pdf |
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ftunigroningenpu:oai:pure.rug.nl:publications/3f230449-e252-4108-a5ca-4d86bd6fd85d 2024-06-02T07:56:30+00:00 Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B Dai, Tianhe Miletic, Nemanja Loos, Katja Elbahri, Mady Abetz, Volker Miletić, Nemanja 2011-02-15 application/pdf https://hdl.handle.net/11370/3f230449-e252-4108-a5ca-4d86bd6fd85d https://research.rug.nl/en/publications/3f230449-e252-4108-a5ca-4d86bd6fd85d https://doi.org/10.1002/macp.201000536 https://pure.rug.nl/ws/files/6759274/2011MacromolChemPhysDai.pdf eng eng https://research.rug.nl/en/publications/3f230449-e252-4108-a5ca-4d86bd6fd85d info:eu-repo/semantics/openAccess Dai , T , Miletic , N , Loos , K , Elbahri , M , Abetz , V & Miletić , N 2011 , ' Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B ' , Macromolecular Chemistry and Physics , vol. 212 , no. 4 , pp. 319-327 . https://doi.org/10.1002/macp.201000536 Candida antarctica lipase B electrospinning enzyme immobilization nanomats poly[acrylonitrile-co-(glycidyl methacrylate)] (PANGMA) MULTIPOINT COVALENT ATTACHMENT GLYCOL DIMETHACRYLATE) RESINS GLUCOSE-OXIDASE HOMOGENEOUS CATALYSTS UNIQUE BIOCATALYSTS POLYMER MEMBRANE ALPHA-AMYLASE CHITOSAN ULTRAFILTRATION article 2011 ftunigroningenpu https://doi.org/10.1002/macp.201000536 2024-05-07T18:47:21Z PANGMA nanofibers and nanomats with fiber diameters of 200-300 nanometers were fabricated by electrospinning. Cal-B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal-B-immobilized PANGMA nanomats were assayed and compared with the free Cal-B. The observed Cal-B loading on these nanomats is up to approximate to 50mg . g(-1), and their hydrolytic activity is up to approximate to 2 500 nmol . min(-1) . mg(-1), much higher than free enzyme powder and also slightly higher than Novozyme 435. Cal-B immobilized PANGMA nanomats have better reusability, thermal stability, and storage ability than free Cal-B. They retain over 50% of their initial activity after 15 cycles, over 65% after 10 h heat incubation, and over 75% after 30 d storage. Article in Journal/Newspaper Antarc* Antarctica University of Groningen research database Macromolecular Chemistry and Physics 212 4 319 327 |
institution |
Open Polar |
collection |
University of Groningen research database |
op_collection_id |
ftunigroningenpu |
language |
English |
topic |
Candida antarctica lipase B electrospinning enzyme immobilization nanomats poly[acrylonitrile-co-(glycidyl methacrylate)] (PANGMA) MULTIPOINT COVALENT ATTACHMENT GLYCOL DIMETHACRYLATE) RESINS GLUCOSE-OXIDASE HOMOGENEOUS CATALYSTS UNIQUE BIOCATALYSTS POLYMER MEMBRANE ALPHA-AMYLASE CHITOSAN ULTRAFILTRATION |
spellingShingle |
Candida antarctica lipase B electrospinning enzyme immobilization nanomats poly[acrylonitrile-co-(glycidyl methacrylate)] (PANGMA) MULTIPOINT COVALENT ATTACHMENT GLYCOL DIMETHACRYLATE) RESINS GLUCOSE-OXIDASE HOMOGENEOUS CATALYSTS UNIQUE BIOCATALYSTS POLYMER MEMBRANE ALPHA-AMYLASE CHITOSAN ULTRAFILTRATION Dai, Tianhe Miletic, Nemanja Loos, Katja Elbahri, Mady Abetz, Volker Miletić, Nemanja Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
topic_facet |
Candida antarctica lipase B electrospinning enzyme immobilization nanomats poly[acrylonitrile-co-(glycidyl methacrylate)] (PANGMA) MULTIPOINT COVALENT ATTACHMENT GLYCOL DIMETHACRYLATE) RESINS GLUCOSE-OXIDASE HOMOGENEOUS CATALYSTS UNIQUE BIOCATALYSTS POLYMER MEMBRANE ALPHA-AMYLASE CHITOSAN ULTRAFILTRATION |
description |
PANGMA nanofibers and nanomats with fiber diameters of 200-300 nanometers were fabricated by electrospinning. Cal-B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal-B-immobilized PANGMA nanomats were assayed and compared with the free Cal-B. The observed Cal-B loading on these nanomats is up to approximate to 50mg . g(-1), and their hydrolytic activity is up to approximate to 2 500 nmol . min(-1) . mg(-1), much higher than free enzyme powder and also slightly higher than Novozyme 435. Cal-B immobilized PANGMA nanomats have better reusability, thermal stability, and storage ability than free Cal-B. They retain over 50% of their initial activity after 15 cycles, over 65% after 10 h heat incubation, and over 75% after 30 d storage. |
format |
Article in Journal/Newspaper |
author |
Dai, Tianhe Miletic, Nemanja Loos, Katja Elbahri, Mady Abetz, Volker Miletić, Nemanja |
author_facet |
Dai, Tianhe Miletic, Nemanja Loos, Katja Elbahri, Mady Abetz, Volker Miletić, Nemanja |
author_sort |
Dai, Tianhe |
title |
Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
title_short |
Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
title_full |
Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
title_fullStr |
Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
title_full_unstemmed |
Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
title_sort |
electrospinning of poly[acrylonitrile-co-(glycidyl methacrylate)] nanofibrous mats for the immobilization of candida antarctica lipase b |
publishDate |
2011 |
url |
https://hdl.handle.net/11370/3f230449-e252-4108-a5ca-4d86bd6fd85d https://research.rug.nl/en/publications/3f230449-e252-4108-a5ca-4d86bd6fd85d https://doi.org/10.1002/macp.201000536 https://pure.rug.nl/ws/files/6759274/2011MacromolChemPhysDai.pdf |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Dai , T , Miletic , N , Loos , K , Elbahri , M , Abetz , V & Miletić , N 2011 , ' Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B ' , Macromolecular Chemistry and Physics , vol. 212 , no. 4 , pp. 319-327 . https://doi.org/10.1002/macp.201000536 |
op_relation |
https://research.rug.nl/en/publications/3f230449-e252-4108-a5ca-4d86bd6fd85d |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1002/macp.201000536 |
container_title |
Macromolecular Chemistry and Physics |
container_volume |
212 |
container_issue |
4 |
container_start_page |
319 |
op_container_end_page |
327 |
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1800756524690178048 |