Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B

• Published in: Macromolecular Chemistry and Physics
• Main Authors: Dai, Tianhe, Miletic, Nemanja, Loos, Katja, Elbahri, Mady, Abetz, Volker, Miletić, Nemanja
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2011
• Subjects: Candida antarctica lipase B; electrospinning; enzyme immobilization; nanomats; poly[acrylonitrile-co-(glycidyl methacrylate)] (PANGMA); MULTIPOINT COVALENT ATTACHMENT; GLYCOL DIMETHACRYLATE) RESINS; GLUCOSE-OXIDASE; HOMOGENEOUS CATALYSTS; UNIQUE BIOCATALYSTS; POLYMER MEMBRANE; ALPHA-AMYLASE; CHITOSAN; ULTRAFILTRATION; Antarc*; Antarctica
• Online Access: https://hdl.handle.net/11370/3f230449-e252-4108-a5ca-4d86bd6fd85d; https://research.rug.nl/en/publications/3f230449-e252-4108-a5ca-4d86bd6fd85d; https://doi.org/10.1002/macp.201000536; https://pure.rug.nl/ws/files/6759274/2011MacromolChemPhysDai.pdf

PANGMA nanofibers and nanomats with fiber diameters of 200-300 nanometers were fabricated by electrospinning. Cal-B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal-B-immobilized PANGMA nanomats were assayed and compared with the free Cal-B. The observed Cal-B loading on these nanomats is up to approximate to 50mg . g(-1), and their hydrolytic activity is up to approximate to 2 500 nmol . min(-1) . mg(-1), much higher than free enzyme powder and also slightly higher than Novozyme 435. Cal-B immobilized PANGMA nanomats have better reusability, thermal stability, and storage ability than free Cal-B. They retain over 50% of their initial activity after 15 cycles, over 65% after 10 h heat incubation, and over 75% after 30 d storage.