Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B

PANGMA nanofibers and nanomats with fiber diameters of 200-300 nanometers were fabricated by electrospinning. Cal-B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal-B-immobilized PANGMA nanomats were assayed and compared with th...

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Bibliographic Details
Published in:Macromolecular Chemistry and Physics
Main Authors: Dai, Tianhe, Miletic, Nemanja, Loos, Katja, Elbahri, Mady, Abetz, Volker, Miletić, Nemanja
Format: Article in Journal/Newspaper
Language:English
Published: 2011
Subjects:
Candida antarctica lipase B
electrospinning
enzyme immobilization
nanomats
poly[acrylonitrile-co-(glycidyl methacrylate)] (PANGMA)
MULTIPOINT COVALENT ATTACHMENT
GLYCOL DIMETHACRYLATE) RESINS
GLUCOSE-OXIDASE
HOMOGENEOUS CATALYSTS
UNIQUE BIOCATALYSTS
POLYMER MEMBRANE
ALPHA-AMYLASE
CHITOSAN
ULTRAFILTRATION
Antarc*
Antarctica
Online Access:https://hdl.handle.net/11370/3f230449-e252-4108-a5ca-4d86bd6fd85d
https://research.rug.nl/en/publications/3f230449-e252-4108-a5ca-4d86bd6fd85d
https://doi.org/10.1002/macp.201000536
https://pure.rug.nl/ws/files/6759274/2011MacromolChemPhysDai.pdf
Description
Summary:PANGMA nanofibers and nanomats with fiber diameters of 200-300 nanometers were fabricated by electrospinning. Cal-B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal-B-immobilized PANGMA nanomats were assayed and compared with the free Cal-B. The observed Cal-B loading on these nanomats is up to approximate to 50mg . g(-1), and their hydrolytic activity is up to approximate to 2 500 nmol . min(-1) . mg(-1), much higher than free enzyme powder and also slightly higher than Novozyme 435. Cal-B immobilized PANGMA nanomats have better reusability, thermal stability, and storage ability than free Cal-B. They retain over 50% of their initial activity after 15 cycles, over 65% after 10 h heat incubation, and over 75% after 30 d storage.

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