Electrospinning of Poly[acrylonitrile-co-(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B
PANGMA nanofibers and nanomats with fiber diameters of 200-300 nanometers were fabricated by electrospinning. Cal-B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal-B-immobilized PANGMA nanomats were assayed and compared with th...
Published in: | Macromolecular Chemistry and Physics |
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Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2011
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Subjects: | |
Online Access: | https://hdl.handle.net/11370/3f230449-e252-4108-a5ca-4d86bd6fd85d https://research.rug.nl/en/publications/3f230449-e252-4108-a5ca-4d86bd6fd85d https://doi.org/10.1002/macp.201000536 https://pure.rug.nl/ws/files/6759274/2011MacromolChemPhysDai.pdf |
Summary: | PANGMA nanofibers and nanomats with fiber diameters of 200-300 nanometers were fabricated by electrospinning. Cal-B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal-B-immobilized PANGMA nanomats were assayed and compared with the free Cal-B. The observed Cal-B loading on these nanomats is up to approximate to 50mg . g(-1), and their hydrolytic activity is up to approximate to 2 500 nmol . min(-1) . mg(-1), much higher than free enzyme powder and also slightly higher than Novozyme 435. Cal-B immobilized PANGMA nanomats have better reusability, thermal stability, and storage ability than free Cal-B. They retain over 50% of their initial activity after 15 cycles, over 65% after 10 h heat incubation, and over 75% after 30 d storage. |
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