Sexing Free-Ranging Brown Bears Ursus-Arctos Using Hairs Found In The Field
Times Cited: 58 International audience As an aid to the management of the Pyrenean population of the brown bear Ursus arctos, a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and tested using hairs found in the field as a source of DNA. This method i...
Published in: | Molecular Ecology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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ftunigrenoble:oai:HAL:halsde-00281064v1 2024-05-12T08:12:13+00:00 Sexing Free-Ranging Brown Bears Ursus-Arctos Using Hairs Found In The Field Taberlet, P. Mattock, H. Duboispaganon, C. Bouvet, J. Laboratoire d'Ecologie Alpine (LECA) Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS) 1993 https://hal.science/halsde-00281064 https://doi.org/10.1111/j.1365-294X.1993.tb00033.x en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-294X.1993.tb00033.x halsde-00281064 https://hal.science/halsde-00281064 doi:10.1111/j.1365-294X.1993.tb00033.x ISSN: 0962-1083 EISSN: 1365-294X Molecular Ecology https://hal.science/halsde-00281064 Molecular Ecology, 1993, 2 (6), pp.399-403. ⟨10.1111/j.1365-294X.1993.tb00033.x⟩ HAIR POLYMERASE CHAIN REACTION SEX IDENTIFICATION SRY GENE URSUS-ARCTOS Y-CHROMOSOME [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment info:eu-repo/semantics/article Journal articles 1993 ftunigrenoble https://doi.org/10.1111/j.1365-294X.1993.tb00033.x 2024-04-18T03:31:01Z Times Cited: 58 International audience As an aid to the management of the Pyrenean population of the brown bear Ursus arctos, a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and tested using hairs found in the field as a source of DNA. This method involves a two-step polymerase chain reaction (PCR) which allows the detection of a very small amount of DNA, probably a single SRY gene molecule. The sex can reliably be identified using about 50pg of DNA extract as template. It is possible that this approach could, with adjustments, be used to identify the sex of other species of eutherian mammals. Article in Journal/Newspaper Ursus arctos Université Grenoble Alpes: HAL Molecular Ecology 2 6 399 403 |
institution |
Open Polar |
collection |
Université Grenoble Alpes: HAL |
op_collection_id |
ftunigrenoble |
language |
English |
topic |
HAIR POLYMERASE CHAIN REACTION SEX IDENTIFICATION SRY GENE URSUS-ARCTOS Y-CHROMOSOME [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment |
spellingShingle |
HAIR POLYMERASE CHAIN REACTION SEX IDENTIFICATION SRY GENE URSUS-ARCTOS Y-CHROMOSOME [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment Taberlet, P. Mattock, H. Duboispaganon, C. Bouvet, J. Sexing Free-Ranging Brown Bears Ursus-Arctos Using Hairs Found In The Field |
topic_facet |
HAIR POLYMERASE CHAIN REACTION SEX IDENTIFICATION SRY GENE URSUS-ARCTOS Y-CHROMOSOME [SDE.BE]Environmental Sciences/Biodiversity and Ecology [SDV.BID]Life Sciences [q-bio]/Biodiversity [SDV.EE]Life Sciences [q-bio]/Ecology environment |
description |
Times Cited: 58 International audience As an aid to the management of the Pyrenean population of the brown bear Ursus arctos, a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and tested using hairs found in the field as a source of DNA. This method involves a two-step polymerase chain reaction (PCR) which allows the detection of a very small amount of DNA, probably a single SRY gene molecule. The sex can reliably be identified using about 50pg of DNA extract as template. It is possible that this approach could, with adjustments, be used to identify the sex of other species of eutherian mammals. |
author2 |
Laboratoire d'Ecologie Alpine (LECA) Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS) |
format |
Article in Journal/Newspaper |
author |
Taberlet, P. Mattock, H. Duboispaganon, C. Bouvet, J. |
author_facet |
Taberlet, P. Mattock, H. Duboispaganon, C. Bouvet, J. |
author_sort |
Taberlet, P. |
title |
Sexing Free-Ranging Brown Bears Ursus-Arctos Using Hairs Found In The Field |
title_short |
Sexing Free-Ranging Brown Bears Ursus-Arctos Using Hairs Found In The Field |
title_full |
Sexing Free-Ranging Brown Bears Ursus-Arctos Using Hairs Found In The Field |
title_fullStr |
Sexing Free-Ranging Brown Bears Ursus-Arctos Using Hairs Found In The Field |
title_full_unstemmed |
Sexing Free-Ranging Brown Bears Ursus-Arctos Using Hairs Found In The Field |
title_sort |
sexing free-ranging brown bears ursus-arctos using hairs found in the field |
publisher |
HAL CCSD |
publishDate |
1993 |
url |
https://hal.science/halsde-00281064 https://doi.org/10.1111/j.1365-294X.1993.tb00033.x |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
ISSN: 0962-1083 EISSN: 1365-294X Molecular Ecology https://hal.science/halsde-00281064 Molecular Ecology, 1993, 2 (6), pp.399-403. ⟨10.1111/j.1365-294X.1993.tb00033.x⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-294X.1993.tb00033.x halsde-00281064 https://hal.science/halsde-00281064 doi:10.1111/j.1365-294X.1993.tb00033.x |
op_doi |
https://doi.org/10.1111/j.1365-294X.1993.tb00033.x |
container_title |
Molecular Ecology |
container_volume |
2 |
container_issue |
6 |
container_start_page |
399 |
op_container_end_page |
403 |
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1798834513828118528 |