Sexing Free-Ranging Brown Bears Ursus-Arctos Using Hairs Found In The Field

Times Cited: 58 International audience As an aid to the management of the Pyrenean population of the brown bear Ursus arctos, a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and tested using hairs found in the field as a source of DNA. This method i...

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Bibliographic Details
Published in:Molecular Ecology
Main Authors: Taberlet, P., Mattock, H., Duboispaganon, C., Bouvet, J.
Other Authors: Laboratoire d'Ecologie Alpine (LECA), Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry )-Centre National de la Recherche Scientifique (CNRS)
Format: Article in Journal/Newspaper
Language:English
Published: HAL CCSD 1993
Subjects:
HAIR
POLYMERASE CHAIN REACTION
SEX IDENTIFICATION
SRY GENE
URSUS-ARCTOS
Y-CHROMOSOME
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
[SDV.BID]Life Sciences [q-bio]/Biodiversity
[SDV.EE]Life Sciences [q-bio]/Ecology
environment
Ursus arctos
Online Access:https://hal.science/halsde-00281064
https://doi.org/10.1111/j.1365-294X.1993.tb00033.x
Description
Summary:Times Cited: 58 International audience As an aid to the management of the Pyrenean population of the brown bear Ursus arctos, a sexing method based on the amplification of a Y chromosome specific sequence has been developed, and tested using hairs found in the field as a source of DNA. This method involves a two-step polymerase chain reaction (PCR) which allows the detection of a very small amount of DNA, probably a single SRY gene molecule. The sex can reliably be identified using about 50pg of DNA extract as template. It is possible that this approach could, with adjustments, be used to identify the sex of other species of eutherian mammals.

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