Influence of phosphate anions on the stability of immobilized enzymes. Effect of enzyme nature, immobilization protocol and inactivation conditions

• Published in: Process Biochemistry
• Main Authors: Kornecki, Jakub F., Carballares, Diego, Sterlinga, Roberto Morellon, Siar, El Hocine, Kashefi, Saeid, Chafiaa, Mazri, Peña, Sara Arana, Rios, Nathalia Saraiva, Gonçalves, Luciana Rocha Barros, Lafuente, Roberto Fernandez
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2021
• Subjects: Lipase interfacial activation; Enzyme destabilization; Immobilized enzyme stability; Buffers and enzyme stability; Tuning enzyme stability by immobilization; Rugosa; Antarc*; Antarctica
• Online Access: https://repositorio.ufrn.br/handle/123456789/45070; https://doi.org/10.1016/j.procbio.2020.02.025

A destabilizing effect at pH 7 of sodium phosphate on several lipases immobilized via interfacial activation is shown in this work. This paper investigates if this destabilizing effect is extended to other inactivation conditions, immobilization protocols or even other immobilized enzymes (ficin, tr...