Nanopartículas de poli-hidroxibutirato-co-valerato como suporte para a imobilização da lipase de Candida antarctica fração B

• Published in: Química Nova
• Main Authors: Fernandes, Ilizandra A., Nyari, Nádia L. D., Oliveira, José Vladimir de, Rigo, Elisandra, Souza, Maria Cristiane M. de, Gonçalves, Luciana R. B., Pergher, Sibele Berenice Castellã, Oliveira, Débora de
• Format: Article in Journal/Newspaper
• Language: Portuguese
• Published: Sociedade Brasileira de Química 2014
• Subjects: Nanoparticles; Immobilization; Lipases; Antarc*; Antarctica
• Online Access: https://repositorio.ufrn.br/jspui/handle/123456789/29631; https://doi.org/10.5935/0100-4042.20140055

This work evaluates the immobilization of Candida antarctica lipase (Fraction B) using poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanoparticles as support. The effects of immobilization time (30-150 min) and pH (5-10) on lipase loading were evaluated. The stability of the immobilized enzyme towards temperature (40, 60, and 80 ºC), reuse and storage (at 4 ºC) were also determined. Furthermore, to assess its potential application in a system of interest, the immobilized lipase was used as a catalyst in the esterification of geraniol with oleic acid. The results indicated a time of 120 minutes and pH of 7 as optimal for immobilization. A 21 hour exposure of the PHBV-lipase derivative to 60 ºC showed a 33% reduction of the initial activity while storage at 4 ºC led to a residual activity (5% of the original activity). The derivative was used without significant loss of activity for 4 successive cycles. The use of the immobilized lipase as a catalyst in the production of geranyl oleate led to about 88% conversion of the initial reactants to products