Isolation and characterization of two different 5S rDNA in Anguilla anguilla and in Anguilla rostrata: Possible markers of evolutionary divergence
We cloned and sequenced the Hae III 350-bp 5S ribosomal DNA (rDNA) band of Anguilla rostrata and designed specific primers from this sequence. Polymerase chain reaction performed with these primers is able to distinguish DNA samples obtained from European ( Anguilla anguilla ) and American ( Anguill...
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ftunicagliariris:oai:iris.unica.it:11584/100258 2024-04-21T07:45:27+00:00 Isolation and characterization of two different 5S rDNA in Anguilla anguilla and in Anguilla rostrata: Possible markers of evolutionary divergence PICHIRI, GIUSEPPINA NIEDDU, MARIELLA CONI, PIERPAOLO SALVADORI, SUSANNA Manconi, S. Casu, C. Mezzanotte, R. Pichiri, Giuseppina Nieddu, Mariella Manconi, S. Casu, C. Coni, Pierpaolo Salvadori, Susanna Mezzanotte, R. 2006 http://hdl.handle.net/11584/100258 https://doi.org/10.1111/j.1471-8286.2006.01394.x eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000239778600017 volume:6 firstpage:638 lastpage:641 numberofpages:4 journal:MOLECULAR ECOLOGY NOTES http://hdl.handle.net/11584/100258 doi:10.1111/j.1471-8286.2006.01394.x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-33747426213 evolutionary divergence Anguilla anguilla and Anguilla rostrata 5S rDNA info:eu-repo/semantics/article 2006 ftunicagliariris https://doi.org/10.1111/j.1471-8286.2006.01394.x 2024-03-25T16:02:00Z We cloned and sequenced the Hae III 350-bp 5S ribosomal DNA (rDNA) band of Anguilla rostrata and designed specific primers from this sequence. Polymerase chain reaction performed with these primers is able to distinguish DNA samples obtained from European ( Anguilla anguilla ) and American ( Anguilla rostrata ) eels. Two amplicons of 1200 bp and 600 bp were obtained, respectively, from A. rostrata and A. anguilla, and the whole 5S rDNA repeated unit from these eels was cloned and sequenced. Southern blot experiments, using four different restriction enzymes and the 5S nontranscribed spacers regions as probe, are able to point out specific diversity in these eels. Article in Journal/Newspaper Anguilla anguilla Università degli Studi di Cagliari: UNICA IRIS Molecular Ecology Notes 6 3 638 641 |
institution |
Open Polar |
collection |
Università degli Studi di Cagliari: UNICA IRIS |
op_collection_id |
ftunicagliariris |
language |
English |
topic |
evolutionary divergence Anguilla anguilla and Anguilla rostrata 5S rDNA |
spellingShingle |
evolutionary divergence Anguilla anguilla and Anguilla rostrata 5S rDNA PICHIRI, GIUSEPPINA NIEDDU, MARIELLA CONI, PIERPAOLO SALVADORI, SUSANNA Manconi, S. Casu, C. Mezzanotte, R. Isolation and characterization of two different 5S rDNA in Anguilla anguilla and in Anguilla rostrata: Possible markers of evolutionary divergence |
topic_facet |
evolutionary divergence Anguilla anguilla and Anguilla rostrata 5S rDNA |
description |
We cloned and sequenced the Hae III 350-bp 5S ribosomal DNA (rDNA) band of Anguilla rostrata and designed specific primers from this sequence. Polymerase chain reaction performed with these primers is able to distinguish DNA samples obtained from European ( Anguilla anguilla ) and American ( Anguilla rostrata ) eels. Two amplicons of 1200 bp and 600 bp were obtained, respectively, from A. rostrata and A. anguilla, and the whole 5S rDNA repeated unit from these eels was cloned and sequenced. Southern blot experiments, using four different restriction enzymes and the 5S nontranscribed spacers regions as probe, are able to point out specific diversity in these eels. |
author2 |
Pichiri, Giuseppina Nieddu, Mariella Manconi, S. Casu, C. Coni, Pierpaolo Salvadori, Susanna Mezzanotte, R. |
format |
Article in Journal/Newspaper |
author |
PICHIRI, GIUSEPPINA NIEDDU, MARIELLA CONI, PIERPAOLO SALVADORI, SUSANNA Manconi, S. Casu, C. Mezzanotte, R. |
author_facet |
PICHIRI, GIUSEPPINA NIEDDU, MARIELLA CONI, PIERPAOLO SALVADORI, SUSANNA Manconi, S. Casu, C. Mezzanotte, R. |
author_sort |
PICHIRI, GIUSEPPINA |
title |
Isolation and characterization of two different 5S rDNA in Anguilla anguilla and in Anguilla rostrata: Possible markers of evolutionary divergence |
title_short |
Isolation and characterization of two different 5S rDNA in Anguilla anguilla and in Anguilla rostrata: Possible markers of evolutionary divergence |
title_full |
Isolation and characterization of two different 5S rDNA in Anguilla anguilla and in Anguilla rostrata: Possible markers of evolutionary divergence |
title_fullStr |
Isolation and characterization of two different 5S rDNA in Anguilla anguilla and in Anguilla rostrata: Possible markers of evolutionary divergence |
title_full_unstemmed |
Isolation and characterization of two different 5S rDNA in Anguilla anguilla and in Anguilla rostrata: Possible markers of evolutionary divergence |
title_sort |
isolation and characterization of two different 5s rdna in anguilla anguilla and in anguilla rostrata: possible markers of evolutionary divergence |
publishDate |
2006 |
url |
http://hdl.handle.net/11584/100258 https://doi.org/10.1111/j.1471-8286.2006.01394.x |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000239778600017 volume:6 firstpage:638 lastpage:641 numberofpages:4 journal:MOLECULAR ECOLOGY NOTES http://hdl.handle.net/11584/100258 doi:10.1111/j.1471-8286.2006.01394.x info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-33747426213 |
op_doi |
https://doi.org/10.1111/j.1471-8286.2006.01394.x |
container_title |
Molecular Ecology Notes |
container_volume |
6 |
container_issue |
3 |
container_start_page |
638 |
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641 |
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