Isolation and characterization of two different 5S rDNA in Anguilla anguilla and in Anguilla rostrata: Possible markers of evolutionary divergence

We cloned and sequenced the Hae III 350-bp 5S ribosomal DNA (rDNA) band of Anguilla rostrata and designed specific primers from this sequence. Polymerase chain reaction performed with these primers is able to distinguish DNA samples obtained from European ( Anguilla anguilla ) and American ( Anguill...

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Bibliographic Details
Published in:Molecular Ecology Notes
Main Authors: PICHIRI, GIUSEPPINA, NIEDDU, MARIELLA, CONI, PIERPAOLO, SALVADORI, SUSANNA, Manconi, S., Casu, C., Mezzanotte, R.
Other Authors: Pichiri, Giuseppina, Nieddu, Mariella, Coni, Pierpaolo, Salvadori, Susanna
Format: Article in Journal/Newspaper
Language:English
Published: 2006
Subjects:
evolutionary divergence
Anguilla anguilla and Anguilla rostrata
5S rDNA
Anguilla anguilla
Online Access:http://hdl.handle.net/11584/100258
https://doi.org/10.1111/j.1471-8286.2006.01394.x
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Summary:We cloned and sequenced the Hae III 350-bp 5S ribosomal DNA (rDNA) band of Anguilla rostrata and designed specific primers from this sequence. Polymerase chain reaction performed with these primers is able to distinguish DNA samples obtained from European ( Anguilla anguilla ) and American ( Anguilla rostrata ) eels. Two amplicons of 1200 bp and 600 bp were obtained, respectively, from A. rostrata and A. anguilla, and the whole 5S rDNA repeated unit from these eels was cloned and sequenced. Southern blot experiments, using four different restriction enzymes and the 5S nontranscribed spacers regions as probe, are able to point out specific diversity in these eels.

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