Evaluation of Argentinean white rot fungi for their ability to produce lignin-modifying enzymes and decolorize industrial dyes

• Language: unknown
• Published: 2004
• Subjects: Coriolus versicolor f. antarcticus; Dye decoloration; Ligninolytic enzymes; White rot fungi; Dyes; Enzymes; Fungi; Manganese compounds; Strain; Decolorizing capacity; Extracellular production; Biotechnology; anthraquinone blue; anthraquinone derivative; azure B; brilliant blue; congo red; dye; fungal enzyme; indigo carmine; industrial enzyme; laccase; lignin; lignin peroxidase; malachite green; manganese peroxidase; unclassified drug; article; controlled study; decolorization; enzyme activity; enzyme assay; enzyme synthesis; fungal strain; fungus culture; nonhuman; Phanerochaete; priority journal; reaction time; solid state fermentation; strain difference; Argentina; Basidiomycota; Biodegradation; Environmental; Cereals; Color; Coloring Agents; Antarc*; antarcticus
• Online Access: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09608524_v94_n2_p169_Levin; https://hdl.handle.net/20.500.12110/paper_09608524_v94_n2_p169_Levin

The decolorizing capacity of 26 white rot fungi from Argentina was investigated. Extracellular production of ligninolytic enzymes by mycelium growing on solid malt extract/glucose medium supplemented with different dyes (Malachite Green, Azure B, Poly R-478, Anthraquinone Blue, Congo Red and Xylidine), dye decolorization and the relationship between these two processes were studied. Only ten strains decolorized all the dyes, all ten strains produced laccase, lignin peroxidase and manganese peroxidase on solid medium. However, six of the strains could not decolorize any of the dyes; all six strains tested negative for lignin peroxidase, and produced less than 0.05 U/g agar of manganese peroxidase. Comparing the isolates with the well-known dye-degrader Phanerochaete chrysosporium, a new fungus was identified: Coriolus versicolor f. antarcticus, potentially a candidate for use in biodecoloration processes. Eighteen day-old cultures of this fungus were able to decolorize in an hour 28%, 30%, 43%, 88% and 98% of Xylidine (24 mg/l), Poly R-478 (75 mg/l), Remazol Brilliant Blue R (9 mg/l), Malachite Green (6 mg/l) and Indigo Carmine (23 mg/l), respectively. Laccase activity was 0.13 U/ml, but neither lignin peroxidase nor manganese peroxidase were detected in the extracellular fluids for that day of incubation. © 2003 Elsevier Ltd. All rights reserved.