The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island

• Main Authors: Ayub, Nicolás Daniel, Pettinari, María Julia, Méndez, Beatriz Silvia
• Language: unknown
• Published: 2007
• Subjects: Antarctica; Genomic islands; Polyhydroxyalkanoates; Pseudomonas; bacterial protein; polyhydroxyalkanoic acid; adaptation syndrome; article; Azotobacter vinelandii; bacterial gene; bacterial genome; bacterial strain; Burkholderia; codon usage; gene insertion sequence; gene sequence; genetic variability; horizontal gene transfer; intron; molecular phylogeny; nucleotide sequence; open reading frame; plasmid; protein secretion; Proteobacteria; sequence homology; unindexed sequence; Acyltransferases; Antarctic Regions; Bacterial Proteins; Base Composition; Base Sequence; Cloning; Molecular; DNA-Binding Proteins; Genes; Bacterial; Hydroxybutyrates; Molecular Sequence Data; Open Reading Frames; Phylogeny; Repressor Proteins; Sequence Alignment; Bacteria (microorganisms); Burkholderiales; Antarctic; Argentina; Antarc*
• Online Access: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0147619X_v58_n3_p240_Ayub; https://hdl.handle.net/20.500.12110/paper_0147619X_v58_n3_p240_Ayub

Pseudomonas sp. 14-3 is an Antarctic bacterium that shows high stress resistance in association with high polyhydroxybutyrate (PHB) production. In this paper genes involved in PHB biosynthesis (phaRBAC) were found within a genomic island named pha-GI. Numerous mobile elements or proteins associated with them, such as an integrase, insertion sequences, a bacterial group II intron, a complete Type I protein secretion system and IncP plasmid-related proteins were detected among the 28 ORFs identified in this large genetic element (32.3 kb). The G + C distribution was not homogeneous, likely reflecting a mosaic structure that contains regions from diverse origins. pha-GI has strong similarities with genomic islands found in diverse Proteobacteria, including Burkholderiales species and Azotobacter vinelandii. The G + C content, phylogeny inference and codon usage analysis showed that the phaBAC cluster itself has a complex mosaic structure and indicated that the phaB and phaC genes were acquired by horizontal transfer, probably derived from Burkholderiales. These results describe for the first time a pha cluster located within a genomic island, and suggest that horizontal transfer of pha genes is a mechanism of adaptability to stress conditions such as those found in the extreme Antarctic environment. © 2007 Elsevier Inc. All rights reserved. Fil:Ayub, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.