Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel
Low egg quality and embryonic survival are critical challenges in aquaculture, where assisted reproduction procedures and other factors may impact egg quality. This includes European eel (Anguilla anguilla), where pituitary extract from carp (CPE) or salmon (SPE) is applied to override a dopaminergi...
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Online Access: | https://hdl.handle.net/11585/903240 https://doi.org/10.1371/journal.pone.0235617 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235617 |
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ftunibolognairis:oai:cris.unibo.it:11585/903240 2024-05-19T07:28:35+00:00 Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel Kottmann, Johanna S Jørgensen, Michelle G P Bertolini, Francesca Loh, Adrian Tomkiewicz, Jonna Kottmann, Johanna S Jørgensen, Michelle G P Bertolini, Francesca Loh, Adrian Tomkiewicz, Jonna 2020 ELETTRONICO https://hdl.handle.net/11585/903240 https://doi.org/10.1371/journal.pone.0235617 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235617 eng eng info:eu-repo/semantics/altIdentifier/pmid/32634160 info:eu-repo/semantics/altIdentifier/wos/WOS:000550645600030 volume:15 issue:7 firstpage:1 lastpage:24 numberofpages:24 journal:PLOS ONE https://hdl.handle.net/11585/903240 doi:10.1371/journal.pone.0235617 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85087668632 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235617 info:eu-repo/semantics/openAccess Anguilla Animal Carp Cyclin A1 Female Fish Protein Gene Expression Regulation Developmental Octamer Transcription Factor-3 Pituitary Gland Pituitary Hormone RNA Messenger Salmon Zygote Embryonic Development Oogenesis info:eu-repo/semantics/article 2020 ftunibolognairis https://doi.org/10.1371/journal.pone.0235617 2024-04-19T00:06:06Z Low egg quality and embryonic survival are critical challenges in aquaculture, where assisted reproduction procedures and other factors may impact egg quality. This includes European eel (Anguilla anguilla), where pituitary extract from carp (CPE) or salmon (SPE) is applied to override a dopaminergic inhibition of the neuroendocrine system, preventing gonadotropin secretion and gonadal development. The present study used either CPE or SPE to induce vitellogenesis in female European eel and compared impacts on egg quality and offspring developmental competence with emphasis on the maternal-to-zygotic transition (MZT). Females treated with SPE produced significantly higher proportions of floating eggs with fewer cleavage abnormalities and higher embryonic survival. These findings related successful embryogenesis to higher abundance of mRNA transcripts of genes involved in cell adhesion, activation of MZT, and immune response (dcbld1,epcam,oct4,igm) throughout embryonic development. The abundance of mRNA transcripts ofcldnd,foxr1,cea,ccna1,ccnb1,ccnb2,zar1,oct4, andnpm2was relatively stable during the first eight hours, followed by a drop during MZT and low levels thereafter, indicating transfer and subsequent clearance of maternal mRNA. mRNA abundance ofzar1,epcam, anddicer1was associated with cleavage abnormalities, while mRNA abundance ofzar1,sox2,foxr1,cldnd,phb2,neurod4, andneurog1(before MZT) was associated with subsequent embryonic survival. In a second pattern, low initial mRNA abundance with an increase during MZT and higher levels persisting thereafter indicating the activation of zygotic transcription. mRNA abundance ofccna1,npm2,oct4,neurod4, andneurog1during later embryonic development was associated with hatch success. A deviating pattern was observed fordcbld1, which mRNA levels followed the maternal-effect gene pattern but only for embryos from SPE treated females. Together, the differences in offspring production and performance reported in this study show that PE composition impacts egg quality ... Article in Journal/Newspaper Anguilla anguilla IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) PLOS ONE 15 7 e0235617 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
op_collection_id |
ftunibolognairis |
language |
English |
topic |
Anguilla Animal Carp Cyclin A1 Female Fish Protein Gene Expression Regulation Developmental Octamer Transcription Factor-3 Pituitary Gland Pituitary Hormone RNA Messenger Salmon Zygote Embryonic Development Oogenesis |
spellingShingle |
Anguilla Animal Carp Cyclin A1 Female Fish Protein Gene Expression Regulation Developmental Octamer Transcription Factor-3 Pituitary Gland Pituitary Hormone RNA Messenger Salmon Zygote Embryonic Development Oogenesis Kottmann, Johanna S Jørgensen, Michelle G P Bertolini, Francesca Loh, Adrian Tomkiewicz, Jonna Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel |
topic_facet |
Anguilla Animal Carp Cyclin A1 Female Fish Protein Gene Expression Regulation Developmental Octamer Transcription Factor-3 Pituitary Gland Pituitary Hormone RNA Messenger Salmon Zygote Embryonic Development Oogenesis |
description |
Low egg quality and embryonic survival are critical challenges in aquaculture, where assisted reproduction procedures and other factors may impact egg quality. This includes European eel (Anguilla anguilla), where pituitary extract from carp (CPE) or salmon (SPE) is applied to override a dopaminergic inhibition of the neuroendocrine system, preventing gonadotropin secretion and gonadal development. The present study used either CPE or SPE to induce vitellogenesis in female European eel and compared impacts on egg quality and offspring developmental competence with emphasis on the maternal-to-zygotic transition (MZT). Females treated with SPE produced significantly higher proportions of floating eggs with fewer cleavage abnormalities and higher embryonic survival. These findings related successful embryogenesis to higher abundance of mRNA transcripts of genes involved in cell adhesion, activation of MZT, and immune response (dcbld1,epcam,oct4,igm) throughout embryonic development. The abundance of mRNA transcripts ofcldnd,foxr1,cea,ccna1,ccnb1,ccnb2,zar1,oct4, andnpm2was relatively stable during the first eight hours, followed by a drop during MZT and low levels thereafter, indicating transfer and subsequent clearance of maternal mRNA. mRNA abundance ofzar1,epcam, anddicer1was associated with cleavage abnormalities, while mRNA abundance ofzar1,sox2,foxr1,cldnd,phb2,neurod4, andneurog1(before MZT) was associated with subsequent embryonic survival. In a second pattern, low initial mRNA abundance with an increase during MZT and higher levels persisting thereafter indicating the activation of zygotic transcription. mRNA abundance ofccna1,npm2,oct4,neurod4, andneurog1during later embryonic development was associated with hatch success. A deviating pattern was observed fordcbld1, which mRNA levels followed the maternal-effect gene pattern but only for embryos from SPE treated females. Together, the differences in offspring production and performance reported in this study show that PE composition impacts egg quality ... |
author2 |
Kottmann, Johanna S Jørgensen, Michelle G P Bertolini, Francesca Loh, Adrian Tomkiewicz, Jonna |
format |
Article in Journal/Newspaper |
author |
Kottmann, Johanna S Jørgensen, Michelle G P Bertolini, Francesca Loh, Adrian Tomkiewicz, Jonna |
author_facet |
Kottmann, Johanna S Jørgensen, Michelle G P Bertolini, Francesca Loh, Adrian Tomkiewicz, Jonna |
author_sort |
Kottmann, Johanna S |
title |
Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel |
title_short |
Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel |
title_full |
Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel |
title_fullStr |
Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel |
title_full_unstemmed |
Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel |
title_sort |
differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in european eel |
publishDate |
2020 |
url |
https://hdl.handle.net/11585/903240 https://doi.org/10.1371/journal.pone.0235617 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235617 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/32634160 info:eu-repo/semantics/altIdentifier/wos/WOS:000550645600030 volume:15 issue:7 firstpage:1 lastpage:24 numberofpages:24 journal:PLOS ONE https://hdl.handle.net/11585/903240 doi:10.1371/journal.pone.0235617 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85087668632 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235617 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0235617 |
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PLOS ONE |
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15 |
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