Differential impacts of carp and salmon pituitary extracts on induced oogenesis, egg quality, molecular ontogeny and embryonic developmental competence in European eel

Low egg quality and embryonic survival are critical challenges in aquaculture, where assisted reproduction procedures and other factors may impact egg quality. This includes European eel (Anguilla anguilla), where pituitary extract from carp (CPE) or salmon (SPE) is applied to override a dopaminergi...

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Bibliographic Details
Published in:PLOS ONE
Main Authors: Kottmann, Johanna S, Jørgensen, Michelle G P, Bertolini, Francesca, Loh, Adrian, Tomkiewicz, Jonna
Format: Article in Journal/Newspaper
Language:English
Published: 2020
Subjects:
Anguilla
Animal
Carp
Cyclin A1
Female
Fish Protein
Gene Expression Regulation
Developmental
Octamer Transcription Factor-3
Pituitary Gland
Pituitary Hormone
RNA
Messenger
Salmon
Zygote
Embryonic Development
Oogenesis
Anguilla anguilla
Online Access:https://hdl.handle.net/11585/903240
https://doi.org/10.1371/journal.pone.0235617
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235617
Description
Summary:Low egg quality and embryonic survival are critical challenges in aquaculture, where assisted reproduction procedures and other factors may impact egg quality. This includes European eel (Anguilla anguilla), where pituitary extract from carp (CPE) or salmon (SPE) is applied to override a dopaminergic inhibition of the neuroendocrine system, preventing gonadotropin secretion and gonadal development. The present study used either CPE or SPE to induce vitellogenesis in female European eel and compared impacts on egg quality and offspring developmental competence with emphasis on the maternal-to-zygotic transition (MZT). Females treated with SPE produced significantly higher proportions of floating eggs with fewer cleavage abnormalities and higher embryonic survival. These findings related successful embryogenesis to higher abundance of mRNA transcripts of genes involved in cell adhesion, activation of MZT, and immune response (dcbld1,epcam,oct4,igm) throughout embryonic development. The abundance of mRNA transcripts ofcldnd,foxr1,cea,ccna1,ccnb1,ccnb2,zar1,oct4, andnpm2was relatively stable during the first eight hours, followed by a drop during MZT and low levels thereafter, indicating transfer and subsequent clearance of maternal mRNA. mRNA abundance ofzar1,epcam, anddicer1was associated with cleavage abnormalities, while mRNA abundance ofzar1,sox2,foxr1,cldnd,phb2,neurod4, andneurog1(before MZT) was associated with subsequent embryonic survival. In a second pattern, low initial mRNA abundance with an increase during MZT and higher levels persisting thereafter indicating the activation of zygotic transcription. mRNA abundance ofccna1,npm2,oct4,neurod4, andneurog1during later embryonic development was associated with hatch success. A deviating pattern was observed fordcbld1, which mRNA levels followed the maternal-effect gene pattern but only for embryos from SPE treated females. Together, the differences in offspring production and performance reported in this study show that PE composition impacts egg quality ...

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