Characterization of a β2 adrenergic receptor protein precursor in the European eel (Anguilla anguilla) and its tissue distribution across silvering

This study provides the characterization and tissue distribution of a β2-AR in the female European eel during silvering, aiming to better understand the adrenergic system involvement in this critical maturation event. A putative β2-AR (ADRB2) mRNA was cloned and sequenced. Amino acid residues and mo...

Full description

Bibliographic Details
Published in:Marine Environmental Research
Main Authors: Franzellitti, Silvia, Kiwan, Alisar, Valbonesi, Paola, Capolupo, Marco, Buratti, Sara, Moon, Thomas W., Fabbri, Elena
Other Authors: Franzellitti, Silvia*
Format: Article in Journal/Newspaper
Language:English
Published: 2018
Subjects:
Adrenergic ligand
Adrenergic receptor
Fish
Gene transcription
Sexual maturation
Silvering
Tissue distribution
Oceanography
Aquatic Science
Pollution
Anguilla anguilla
Online Access:http://hdl.handle.net/11585/636237
https://doi.org/10.1016/j.marenvres.2018.03.006
Description
Summary:This study provides the characterization and tissue distribution of a β2-AR in the female European eel during silvering, aiming to better understand the adrenergic system involvement in this critical maturation event. A putative β2-AR (ADRB2) mRNA was cloned and sequenced. Amino acid residues and motifs important for ligand binding are generally conserved across fish and between fish and mammals, although the occurrence of some sequence variabilities may explain the noted peculiarities of eel AR interaction with pharmacological ligands. The tissue distribution of the ADRB2 gene product was analyzed in five tissues of the eel at different silvering stages and compared with that of the ADRA1 mRNA encoding an α1-AR subtype. On the whole, data suggested that relative ADRA1/ADRB2 tissue expression across silvering is part of the preparatory (molecular) adjustments required to face changes in habitats and migration efforts.

Similar Items