Human antibody recognition of Anisakidae and Trichinella spp. in Greenland.

High levels of total IgE are observed among children in Greenland. To evaluate the extent to which Anisakidae and Trichinella spp. contribute to the high total IgE level, an ELISA and a western blot were developed for the detection of IgG antibodies to Anisakidae, based on excretory/secretory antige...

Full description

Bibliographic Details
Published in:Clinical Microbiology and Infection
Main Authors: Møller, L N, Krause, T Grove, Koch, A, Melbye, M, Kapel, C M O, Petersen, E
Format: Article in Journal/Newspaper
Language:English
Published: 2007
Subjects:
Adolescent
Animals
Antibodies
Helminth
Antibody Specificity
Antigens
Ascaridida Infections
Ascaridoidea
Child
Enzyme-Linked Immunosorbent Assay
Female
Greenland
Humans
Immunoglobulin E
Immunoglobulin G
Larva
Male
Trichinella
Trichinosis
Online Access:https://pure.au.dk/portal/da/publications/human-antibody-recognition-of-anisakidae-and-trichinella-spp-in-greenland(da89f6b0-e602-11dc-9afb-000ea68e967b).html
https://doi.org/10.1111/j.1469-0691.2007.01730.x
Description
Summary:High levels of total IgE are observed among children in Greenland. To evaluate the extent to which Anisakidae and Trichinella spp. contribute to the high total IgE level, an ELISA and a western blot were developed for the detection of IgG antibodies to Anisakidae, based on excretory/secretory antigens from Anisakidae larvae. Western blots with Anisakidae and Trichinella antigens discriminated between Anisakidae and Trichinella infections, enabling cross-reactivity between the two parasite infections to be eliminated. Serum samples from 1012 children in Greenland were analysed for specific antibodies to Anisakidae and Trichinella. Eleven children were IgG-positive for Trichinella and nine were IgG-positive for Anisakidae, indicating a relatively low prevalence of both infections among children in Greenland. Faecal samples from 320 children were also examined for other intestinal parasites. Enterobius vermicularis was found in one sample and Blastocystis hominis in 32 samples, but no other intestinal parasites were identified. In total, 304 children had elevated total IgE levels. There was a significant association between Trichinella seropositivity and high levels of total IgE, but not between Anisakidae seropositivity and total IgE. The data indicate that parasitic infections alone do not explain the high level of total IgE observed among children in Greenland. Udgivelsesdato: 2007-Jul

Similar Items