| Summary: | Udgivelsesdato: May 25 Background: Persistent organic pollutants (POPs) such as polychlorinated dibenzo--dioxins/furans, polychlorinatedbiphenyls (PCBs) and organochlorine pesticides can cause a series of adverse effects on e.g. reproduction in animals andhumans, many of which involve the aryl hydrocarbon receptor (AhR). The aim of the present study was to compare theintegrated serum level of AhR mediated activity among European and Inuit populations, and evaluate whether the activitywas associated to the selected POP markers, 2,2',4,4',5,5'-hexachlorobiphenyl (CB-153) and 1,1-dichloro-2,2-bis(pchlorophenyl)-ethylene ( p,p' -DDE).Methods: The study included 338 males from Greenland (Inuit's), Sweden, Warsaw (Poland) and Kharkiv (Ukraine). TheAhR transactivity of serum extracts alone (AhRag) and competitive AhR activity (AhRcomp) upon co-exposure with2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD) were determined in the lipophilic serum fraction containing the POPs usingthe AhR mediated luciferase reporter Hepa1.12cR cell assay.Results: The European groups showed higher median level of AhR-TEQ (TCDD toxic equivalents) compared to theInuit's, whereas higher incidence of Inuits sample further induced AhRcomp activity. Neither AhRag nor AhR-TEQ werecorrelated to CB-153 or p,p' -DDE for any of the study groups. Multiple regressions showed a significant heterogeneityof association between the CB-153 and the AhRcomp across the study groups, and accordingly a negative associationbetween AhRcomp and CB-153 was found for the Kharkiv group. Conclusion: No consistent correlation between AhR activities and two POP markers was found. Although thedifference of AhRag between European and Inuit men could not be explained by CB-153 or p,p' -DDE levels alone, webelieve that the variation of AhR serum activity reflects different pattern of POP exposure, genetics and/or life stylefactors.
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