Evaluation of two enzyme-linked immunosorbent assays measuring pregnancy-associated glycoproteins in the blood of muskoxen ( Ovibos moschatus )

Pregnancy-associated glycoproteins (PAGs) are expressed by the ruminal placenta, making their detection in blood an accurate indicator of pregnancy. This study aimed to evaluate two commercially available PAG enzyme-linked immunosorbent assays (ELISAs) in muskoxen (Ovibos moschatus). The two tests a...

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Bibliographic Details
Published in:Journal of Zoo and Wildlife Medicine
Main Authors: Greunz, E.M., Stelvig, Mikkel, Grøndahl, Carsten, Schmidt, Niels Martin, Mosbacher, Jesper Bruun, Hansen, Lars Holst, Bertelsen, Mads Frost
Format: Article in Journal/Newspaper
Language:English
Published: 2018
Subjects:
ELISA
Gestation
Muskox
Ovibos moschatus
PAG
Pregnancy diagnosis
muskox
ovibos moschatus
Online Access:https://pure.au.dk/portal/en/publications/10fd288b-ccc1-466d-b277-e50a1a30cca0
https://doi.org/10.1638/2018-0009.1
Description
Summary:Pregnancy-associated glycoproteins (PAGs) are expressed by the ruminal placenta, making their detection in blood an accurate indicator of pregnancy. This study aimed to evaluate two commercially available PAG enzyme-linked immunosorbent assays (ELISAs) in muskoxen (Ovibos moschatus). The two tests are based on the same principles; however, one is evaluated photometrically and the other visually. Sixteen samples covering all trimesters of pregnancy, and 16 nonpregnant samples were included to evaluate test performance. Both tests reliably detected pregnancy. The photometric ELISA showed a sensitivity and specificity of 94% and 100%, respectively. Although the visual ELISA depends on somewhat subjective interpretations, it came up with a sensitivity of 81% and a specificity of 100%, and might thus provide a useful in-house tool when limited laboratory equipment is available. Analysis of additional samples showed consistent results during pregnancy and circulating PAGs for at least 18 days postpartum.

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