| Summary: | Denitrification and nitrification are microbial processes that regulate the cycle of nitrogen and nitrous oxide, which is considered an important greenhouse gas. Rice field soils have been known to have strong denitrifying activities; however, the microorganism structure that is responsible for denitrification and the temporal variation of these communities in the agricultural soils of Ibague (Colombia) is not well known. In this study, the denitrifying community composition was compared between a rice field soil and an uncultivated soil at three different times during the year using a terminal restriction fragment length polymorphism analysis of the nirS functional gene, which codes the enzyme that reduces nitrite, one of the key steps in the denitrification process. The results showed changes in the richness, relative abundance and diversity of the operational taxonomic units between the soils and sampling times. The canonical correspondence analysis indicated that the moisture and the pH were the environmental factors that explained the observed changes in the nirS-type denitrifiers' community composition in the studied soils. La desnitrificación y la nitrificación son procesos microbianos que regulan el ciclo del nitrógeno y del óxido nitroso, el cual es considerado como un importante gas invernadero. Los suelos cultivados con arroz presentan una fuerte actividad desnitrificante, sin embargo, la estructura de los microorganismos responsables de la desnitrificación y la variación temporal de estas comunidades en suelos agrícolas de Ibagué (Colombia) no es muy conocida. En este estudio se comparó la composición de la comunidad desnitrificante entre un suelo cultivado con arroz y uno no cultivado, en tres épocas del año, usando el análisis del polimorfismo de los fragmentos de restricción terminal del gen funcional nirS que codifica la enzima que reduce el nitrito, uno de los pasos claves del proceso de desnitrificación. Los resultados mostraron cambios en la riqueza, abundancia relativa y diversidad de ...
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