Ammonia‐oxidizing archaea and nitrite‐oxidizing nitrospiras in the biofilter of a shrimp recirculating aquaculture system

• Published in: FEMS Microbiology Ecology
• Main Authors: Brown, Monisha N., Briones, Aurelio, Diana, James, Raskin, Lutgarde
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Elsevier 2013
• Subjects: Shrimp Aquaculture; Recirculating Aquaculture System; Ammonia Oxidation; Nitrite Oxidation; N Itrospira; N Itrosopumilus Maritimus; Microbiology and Immunology; Ecology and Evolutionary Biology; Science; Health Sciences; Arctic
• Online Access: http://hdl.handle.net/2027.42/95109; https://doi.org/10.1111/j.1574-6941.2012.01448.x

This study analysed the nitrifier community in the biofilter of a zero discharge, recirculating aquaculture system ( RAS ) for the production of marine shrimp in a low density (low ammonium production) system. The ammonia‐oxidizing populations were examined by targeting 16 S rRNA and amoA genes of ammonia‐oxidizing bacteria ( AOB ) and archaea ( AOA ). The nitrite‐oxidizing bacteria ( NOB ) were investigated by targeting the 16 S rRNA gene. Archaeal amoA genes were more abundant in all compartments of the RAS than bacterial amoA genes. Analysis of bacterial and archaeal amoA gene sequences revealed that most ammonia oxidizers were related to N itrosomonas marina and N itrosopumilus maritimus . The NOB detected were related to N itrospira marina and N itrospira moscoviensis, and Nitrospira marina ‐type NOB were more abundant than N . moscoviensis ‐type NOB . Water quality and biofilm attachment media played a role in the competitiveness of AOA over AOB and Nitrospira marina‐ over N . moscoviensis‐ type NOB . Peer Reviewed http://deepblue.lib.umich.edu/bitstream/2027.42/95109/1/fem1448.pdf