GABA and GABA-transporter (GAT-1) immunoreactivities in the retina of the salmon (Salmo salar L.)

Putative GABAergic elements in the retina of the Atlantic salmon have been identified by immunohistochemistry, utilising polyclonal antisera against γ-aminobutyric acid (GABA) and the GABA transporter GAT-1. Cell types immunoreactive (ir) for GABA comprise horizontal cells, amacrine cells, displaced...

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Bibliographic Details
Published in:Brain Research
Main Authors: Ekström, Peter, Anzelius, Marie
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 1998
Subjects:
Online Access:https://lup.lub.lu.se/record/ffa935ca-4c28-41ce-8569-346b1c01a6be
https://doi.org/10.1016/S0006-8993(98)00994-9
Description
Summary:Putative GABAergic elements in the retina of the Atlantic salmon have been identified by immunohistochemistry, utilising polyclonal antisera against γ-aminobutyric acid (GABA) and the GABA transporter GAT-1. Cell types immunoreactive (ir) for GABA comprise horizontal cells, amacrine cells, displaced amacrine cells in the ganglion cell layer, displaced amacrine cells in the inner plexiform layer (interstitial cells), and Muller cells. In addition, a GABA-immunonegative type of interstitial cell was also identified. In the inner plexiform layer, GABAir fibres were organised in sublayers that were strikingly similar to the sublayering of GAT-1ir fibres. GAT-lir cell bodies comprise amacrine cells and displaced amacrine cells that may represent a subpopulation of the GABAir ones. In view of the very similar sublayering of GABAir and GAT-ir fibres in the IPL we suggest that a similar type of GABA transporter, that can be recognised with antibodies against rat GAT-1, is present at least in the dendrites of all GABAir amacrine cells but is not expressed in the cell bodies of all GABAir cells.