Continuous lipase-catalyzed production of wax ester using silicone tubing
Enzymatic synthesis of cetyl palmitate was performed in a solvent-free system at 65 °C using immobilized Candida antarctica lipase. Batch reactions at controlled water activity showed that the yield could be increased from 88.8 to 99.1% by decreasing the water activity from 1 to 0.05. A continuous r...
| Published in: | Journal of the American Oil Chemists' Society |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
The American Oil Chemists' Society
1999
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| Subjects: | |
| Online Access: | https://lup.lub.lu.se/record/fbb343bb-b125-4a5c-ac08-086c07434892 https://doi.org/10.1007/s11746-999-0190-4 |
| Summary: | Enzymatic synthesis of cetyl palmitate was performed in a solvent-free system at 65 °C using immobilized Candida antarctica lipase. Batch reactions at controlled water activity showed that the yield could be increased from 88.8 to 99.1% by decreasing the water activity from 1 to 0.05. A continuous reactor configuration was constructed, where two tubular reactors were run in sequence with a separation container in between, in which the water phase was separated from the wax ester phase. The reactor was run for 1 wk at low flow rate (0.005 g/min) with very good operational stability and a productivity of 7.2 g d-1 using 0.4 g of biocatalyst. The activity of the individual preparations decreased during operation. The first reactor had only 30% activity left after 1 wk of operation whereas the second reactor showed only a 10% decrease. This difference in enzyme stability is a direct result of the different water activity in the two reactors. |
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