Streamlined Preparation of Immobilized Candida antarctica Lipase B

Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior ac...

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Published in:ACS Omega
Main Authors: Cassimjee, Karim Engelmark, Hendil-Forssell, Peter, Volkov, Alexey, Krog, Anne, Malmo, Jostein, Aune, Trond Erik V, Knecht, Wolfgang, Miskelly, Iain R, Moody, Thomas S, Svedendahl Humble, Maria
Format: Article in Journal/Newspaper
Language:English
Published: The American Chemical Society (ACS) 2017
Subjects:
Biochemistry and Molecular Biology
Antarc*
Antarctica
Online Access:https://lup.lub.lu.se/record/1781d522-9acd-41c5-bdb8-a93944c8bbb7
https://doi.org/10.1021/acsomega.7b01510
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spelling ftulundlup:oai:lup.lub.lu.se:1781d522-9acd-41c5-bdb8-a93944c8bbb7 2023-11-05T03:34:20+01:00 Streamlined Preparation of Immobilized Candida antarctica Lipase B Cassimjee, Karim Engelmark Hendil-Forssell, Peter Volkov, Alexey Krog, Anne Malmo, Jostein Aune, Trond Erik V Knecht, Wolfgang Miskelly, Iain R Moody, Thomas S Svedendahl Humble, Maria 2017-12-06 https://lup.lub.lu.se/record/1781d522-9acd-41c5-bdb8-a93944c8bbb7 https://doi.org/10.1021/acsomega.7b01510 eng eng The American Chemical Society (ACS) https://lup.lub.lu.se/record/1781d522-9acd-41c5-bdb8-a93944c8bbb7 http://dx.doi.org/10.1021/acsomega.7b01510 pmid:30023589 scopus:85059801499 ACS Omega; 2(12), pp 8674-8677 (2017) ISSN: 2470-1343 Biochemistry and Molecular Biology contributiontojournal/article info:eu-repo/semantics/article text 2017 ftulundlup https://doi.org/10.1021/acsomega.7b01510 2023-10-11T22:29:12Z Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435. Article in Journal/Newspaper Antarc* Antarctica Lund University Publications (LUP) ACS Omega 2 12 8674 8677
institution Open Polar
collection Lund University Publications (LUP)
op_collection_id ftulundlup
language English
topic Biochemistry and Molecular Biology
spellingShingle Biochemistry and Molecular Biology
Cassimjee, Karim Engelmark
Hendil-Forssell, Peter
Volkov, Alexey
Krog, Anne
Malmo, Jostein
Aune, Trond Erik V
Knecht, Wolfgang
Miskelly, Iain R
Moody, Thomas S
Svedendahl Humble, Maria
Streamlined Preparation of Immobilized Candida antarctica Lipase B
topic_facet Biochemistry and Molecular Biology
description Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.
format Article in Journal/Newspaper
author Cassimjee, Karim Engelmark
Hendil-Forssell, Peter
Volkov, Alexey
Krog, Anne
Malmo, Jostein
Aune, Trond Erik V
Knecht, Wolfgang
Miskelly, Iain R
Moody, Thomas S
Svedendahl Humble, Maria
author_facet Cassimjee, Karim Engelmark
Hendil-Forssell, Peter
Volkov, Alexey
Krog, Anne
Malmo, Jostein
Aune, Trond Erik V
Knecht, Wolfgang
Miskelly, Iain R
Moody, Thomas S
Svedendahl Humble, Maria
author_sort Cassimjee, Karim Engelmark
title Streamlined Preparation of Immobilized Candida antarctica Lipase B
title_short Streamlined Preparation of Immobilized Candida antarctica Lipase B
title_full Streamlined Preparation of Immobilized Candida antarctica Lipase B
title_fullStr Streamlined Preparation of Immobilized Candida antarctica Lipase B
title_full_unstemmed Streamlined Preparation of Immobilized Candida antarctica Lipase B
title_sort streamlined preparation of immobilized candida antarctica lipase b
publisher The American Chemical Society (ACS)
publishDate 2017
url https://lup.lub.lu.se/record/1781d522-9acd-41c5-bdb8-a93944c8bbb7
https://doi.org/10.1021/acsomega.7b01510
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_source ACS Omega; 2(12), pp 8674-8677 (2017)
ISSN: 2470-1343
op_relation https://lup.lub.lu.se/record/1781d522-9acd-41c5-bdb8-a93944c8bbb7
http://dx.doi.org/10.1021/acsomega.7b01510
pmid:30023589
scopus:85059801499
op_doi https://doi.org/10.1021/acsomega.7b01510
container_title ACS Omega
container_volume 2
container_issue 12
container_start_page 8674
op_container_end_page 8677
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