Streamlined Preparation of Immobilized Candida antarctica Lipase B

Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior ac...

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Bibliographic Details
Published in:ACS Omega
Main Authors: Cassimjee, Karim Engelmark, Hendil-Forssell, Peter, Volkov, Alexey, Krog, Anne, Malmo, Jostein, Aune, Trond Erik V, Knecht, Wolfgang, Miskelly, Iain R, Moody, Thomas S, Svedendahl Humble, Maria
Format: Article in Journal/Newspaper
Language:English
Published: The American Chemical Society (ACS) 2017
Subjects:
Biochemistry and Molecular Biology
Antarc*
Antarctica
Online Access:https://lup.lub.lu.se/record/1781d522-9acd-41c5-bdb8-a93944c8bbb7
https://doi.org/10.1021/acsomega.7b01510
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Summary:Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.

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