Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles
The immobilization of lipase B from Candida antarctica(CalB) within micronsized poly-N-Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determining the embe...
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ftulbdarmstadt:oai:tuprints.ulb.tu-darmstadt.de:19949 2023-09-05T13:13:16+02:00 Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles Gawlitza, Kornelia Wu, Changzhu Georgieva, Radostina Ansorge-Schumacher, Marion Klitzing, Regine von 2022 text http://tuprints.ulb.tu-darmstadt.de/19949/ https://tuprints.ulb.tu-darmstadt.de/19949/1/10.1524_zpch.2012.0271.pdf https://doi.org/10.26083/tuprints-00019949 en eng De Gruyter https://tuprints.ulb.tu-darmstadt.de/19949/1/10.1524_zpch.2012.0271.pdf Gawlitza, Kornelia <http://tuprints.ulb.tu-darmstadt.de/view/person/Gawlitza=3AKornelia=3A=3A.html> Wu, Changzhu <http://tuprints.ulb.tu-darmstadt.de/view/person/Wu=3AChangzhu=3A=3A.html> Georgieva, Radostina <http://tuprints.ulb.tu-darmstadt.de/view/person/Georgieva=3ARadostina=3A=3A.html> Ansorge-Schumacher, Marion <http://tuprints.ulb.tu-darmstadt.de/view/person/Ansorge-Schumacher=3AMarion=3A=3A.html> Klitzing, Regine von <http://tuprints.ulb.tu-darmstadt.de/view/person/Klitzing=3ARegine_von=3A=3A.html> (2022):Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles. (Publisher's Version)In: Zeitschrift für Physikalische Chemie, 226 (7-8), pp. 749-759. De Gruyter, ISSN 0942-9352, e-ISSN 2196-7156, DOI:10.26083/tuprints-00019949 <https://doi.org/10.26083/tuprints-00019949>, [Article] In Copyright info:eu-repo/semantics/openAccess Article NonPeerReviewed info:eu-repo/semantics/article 2022 ftulbdarmstadt https://doi.org/10.26083/tuprints-00019949 2023-08-22T19:48:12Z The immobilization of lipase B from Candida antarctica(CalB) within micronsized poly-N-Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determining the embedded amount of CalB within the polymer network, an enhanced specificactivity inn-hexane was obtained. Due to the thermoresponsibility of the polymer particles, the activity reaction was done at 25°C and 50°C. The results presented show that the reversiblecollapse of the microgel leads to a decreased activity with increasing temperature. Hence, p-NIPAMmicrogels display a good opportunity to tailor the activity of CalB. An interesting side effect is that CalB presents a suitable probe to estimate the mesh size of the polymer network, since itpenetrates in the unlabeled form but not after labeling with FITC. Article in Journal/Newspaper Antarc* Antarctica TU Darmstadt: tuprints |
institution |
Open Polar |
collection |
TU Darmstadt: tuprints |
op_collection_id |
ftulbdarmstadt |
language |
English |
description |
The immobilization of lipase B from Candida antarctica(CalB) within micronsized poly-N-Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determining the embedded amount of CalB within the polymer network, an enhanced specificactivity inn-hexane was obtained. Due to the thermoresponsibility of the polymer particles, the activity reaction was done at 25°C and 50°C. The results presented show that the reversiblecollapse of the microgel leads to a decreased activity with increasing temperature. Hence, p-NIPAMmicrogels display a good opportunity to tailor the activity of CalB. An interesting side effect is that CalB presents a suitable probe to estimate the mesh size of the polymer network, since itpenetrates in the unlabeled form but not after labeling with FITC. |
format |
Article in Journal/Newspaper |
author |
Gawlitza, Kornelia Wu, Changzhu Georgieva, Radostina Ansorge-Schumacher, Marion Klitzing, Regine von |
spellingShingle |
Gawlitza, Kornelia Wu, Changzhu Georgieva, Radostina Ansorge-Schumacher, Marion Klitzing, Regine von Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
author_facet |
Gawlitza, Kornelia Wu, Changzhu Georgieva, Radostina Ansorge-Schumacher, Marion Klitzing, Regine von |
author_sort |
Gawlitza, Kornelia |
title |
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
title_short |
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
title_full |
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
title_fullStr |
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
title_full_unstemmed |
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
title_sort |
temperature controlled activity of lipase b from candida antarctica after immobilization within p-nipam microgel particles |
publisher |
De Gruyter |
publishDate |
2022 |
url |
http://tuprints.ulb.tu-darmstadt.de/19949/ https://tuprints.ulb.tu-darmstadt.de/19949/1/10.1524_zpch.2012.0271.pdf https://doi.org/10.26083/tuprints-00019949 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://tuprints.ulb.tu-darmstadt.de/19949/1/10.1524_zpch.2012.0271.pdf Gawlitza, Kornelia <http://tuprints.ulb.tu-darmstadt.de/view/person/Gawlitza=3AKornelia=3A=3A.html> Wu, Changzhu <http://tuprints.ulb.tu-darmstadt.de/view/person/Wu=3AChangzhu=3A=3A.html> Georgieva, Radostina <http://tuprints.ulb.tu-darmstadt.de/view/person/Georgieva=3ARadostina=3A=3A.html> Ansorge-Schumacher, Marion <http://tuprints.ulb.tu-darmstadt.de/view/person/Ansorge-Schumacher=3AMarion=3A=3A.html> Klitzing, Regine von <http://tuprints.ulb.tu-darmstadt.de/view/person/Klitzing=3ARegine_von=3A=3A.html> (2022):Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles. (Publisher's Version)In: Zeitschrift für Physikalische Chemie, 226 (7-8), pp. 749-759. De Gruyter, ISSN 0942-9352, e-ISSN 2196-7156, DOI:10.26083/tuprints-00019949 <https://doi.org/10.26083/tuprints-00019949>, [Article] |
op_rights |
In Copyright info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.26083/tuprints-00019949 |
_version_ |
1776204369359798272 |