Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles
The immobilization of lipase B from Candida antarctica(CalB) within micronsized poly-N-Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determining the embe...
| Main Authors: | , , , , |
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| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
De Gruyter
2022
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| Subjects: | |
| Online Access: | http://tuprints.ulb.tu-darmstadt.de/19949/ https://tuprints.ulb.tu-darmstadt.de/19949/1/10.1524_zpch.2012.0271.pdf https://doi.org/10.26083/tuprints-00019949 |
| Summary: | The immobilization of lipase B from Candida antarctica(CalB) within micronsized poly-N-Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determining the embedded amount of CalB within the polymer network, an enhanced specificactivity inn-hexane was obtained. Due to the thermoresponsibility of the polymer particles, the activity reaction was done at 25°C and 50°C. The results presented show that the reversiblecollapse of the microgel leads to a decreased activity with increasing temperature. Hence, p-NIPAMmicrogels display a good opportunity to tailor the activity of CalB. An interesting side effect is that CalB presents a suitable probe to estimate the mesh size of the polymer network, since itpenetrates in the unlabeled form but not after labeling with FITC. |
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