A laboratory study on O-2 dynamics and photosynthesis in ice algal communities:quantification by microsensors,O-2 exchange rates, C-14 incubations and a PAM fluorometer
The present study compared O-2 exchange measurements, fluorometry and C-14 incubations for measuring productivity of sea-ice algae. It is demonstrated that brine leaking out of ice during freezing conditions is supersaturated with respect to O-2, while the melting water released during thawing is un...
| Published in: | Aquatic Microbial Ecology |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
2002
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| Subjects: | |
| Online Access: | https://pure.uhi.ac.uk/en/publications/4e2b26a0-73dd-4d61-b6c6-0fef72c55acd https://doi.org/10.3354/ame027301 |
| Summary: | The present study compared O-2 exchange measurements, fluorometry and C-14 incubations for measuring productivity of sea-ice algae. It is demonstrated that brine leaking out of ice during freezing conditions is supersaturated with respect to O-2, while the melting water released during thawing is undersaturated with O-2, This results in a highly variable O-2 concentration at the ice-water interface during expanding or shrinking of sea ice. Consequently, great care should be taken when inferring biological activity from O-2 exchange rates, whether they are obtained via microprofiles, bulk incubations or direct measurement Of O-2 Concentrations in water below sea ice. Accounting for the O-2 dynamics related to changes in sea-ice structure, the photosynthetic activity of a mixed ice algal culture, as measured by net O-2 exchange rates and C-14 incubation, co-varied as a function of biomass and irradiance. The ratio between the net O-2 efflux and the gross C fixation rate (the photosynthetic quotient) was similar for frozen and non-frozen ice algal communities and averaged 1.43 +/- 0.48, Quantification of the relative electron transport rate (ETR) from photosystem II (PSII) and the minimum fluorescence yield (F-0) by a pulse-amplitude-modulated (PAM) fluorometer offers a fast, simple and non-invasive approach for estimating activity and biomass of ice algal communities. The relative ETR measurements correlated with the other 2 measures for primary production. However, the correlation was non-linear, leading to a poor resolution of the fluorometer approach at higher photosynthetic activities, A similar observation was made for the correlation between measurements and the concentration of chlorophyll a. |
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