Growth of Piscirickettsia salmonis to high Titers in insect tissue culture cells

Piscirickettsia salmonis was grown in established insect, frog, and fish tissue culture cells. The yield of P. salmonis in Sf21 cells was up to 100 times that obtained in CHSE-214 cells, and virulence for Atlantic salmon was retained. The ceiling temperature for growth of P. salmonis in Sf21 cells w...

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Bibliographic Details
Published in:Infection and Immunity
Main Authors: Birkbeck, T.Harry, Griffen, Angela A., Reid, Helen I., Laidler, L. Anthony, Wadsworth, Simon
Format: Article in Journal/Newspaper
Language:unknown
Published: American Society for Microbiology 2004
Subjects:
Online Access:http://eprints.gla.ac.uk/21449/
Description
Summary:Piscirickettsia salmonis was grown in established insect, frog, and fish tissue culture cells. The yield of P. salmonis in Sf21 cells was up to 100 times that obtained in CHSE-214 cells, and virulence for Atlantic salmon was retained. The ceiling temperature for growth of P. salmonis in Sf21 cells was 24°C.