| Summary: | Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia Química, Florianópolis, 2018. Abstract : Many sectors of industry, mainly food, cosmetics and pharmaceutics, have increased their interest in esters due to their flavor property. Flavor esters that possess an aromatic ring in their molecular structure are also known as aromatic esters. These esters are widely found in nature (fruits and plants) and the synthetic (i.e. via chemical) and natural routes (i.e. via direct extraction from nature or via biotechnology) are suitable for their biocatalysis. Enzyme-catalyzed reactions are the most economical approach to reach final green products with no toxicity and no harm to human health. The study of the process parameters and their interaction are very important to understand the system optimization and achieve the maximum reaction yield to scale up. Benzyl propionate and benzyl butyrate are aromatic esters that possess a fruity and floral odor and are usually found in nature in the composition of some fruits like plums and melons. This work aimed for the benzyl esters biotechnological synthesis by esterification of benzyl alcohol and acid using a new immobilized enzyme preparation with low-cost material from Candida antarctica fraction B (NS 88011) and three commercial immobilized lipases (Novozym 435, Lipozyme TL-IM and Lipozyme RM-IM) from Novozymes®. Novozym 435 had the best performance even when the solvent tert-butanol was absent of the reaction medium. Results from a 2² factorial design showed that an increase in the enzyme amount led to a higher ester conversion, even when the temperature was kept at the low value. Currently, no research had synthesized successfully benzyl propionate via esterification mediated by lipases. Aromatic esters can present some biological activities, in addition to their fragrances. The antimicrobial activity of benzyl butyrate was tested, and the results showed a good performance, indicating that a low ...
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