In vitro intestinal absorption of amino acid mixtures extracted from codfish (Gadus morhua L.) salting wastewater

This study reports on the intestinal permeability of salt-containing mixtures of amino acids extracted from codfish salting wastewater. Permeability was evaluated in vitro using the Caco-2 cell line model; cell integrity during exposure to mixtures of amino acids was estimated by measuring the trans...

Full description

Bibliographic Details
Published in:International Journal of Food Science & Technology
Main Authors: Ferraro, Vincenza, Jorge, Ruben Ferreira, Cruz, Isabel B., Antunes, Filipa, Sarmento, Bruno, Castro, Paula M.L., Pintado, Manuela E.
Format: Article in Journal/Newspaper
Language:English
Published: 2014
Subjects:
Online Access:https://ciencia.ucp.pt/en/publications/7dbdc411-dee7-4936-8dbf-2f2bd811f7a2
https://doi.org/10.1111/ijfs.12269
http://www.scopus.com/inward/record.url?scp=84890426132&partnerID=8YFLogxK
http://hdl.handle.net/10400.14/14429
Description
Summary:This study reports on the intestinal permeability of salt-containing mixtures of amino acids extracted from codfish salting wastewater. Permeability was evaluated in vitro using the Caco-2 cell line model; cell integrity during exposure to mixtures of amino acids was estimated by measuring the transepithelial electrical resistance (TEER). The effect of salt (NaCl) on the permeability and on the intestinal cell's integrity was also examined. Permeation rate (i.e. transport) was ≥95% for all amino acids except for creatine, for which it was 6%. Values for apparent permeability coefficients, Papp > 10-5 cm s-1, for mixture with isotonic concentration of NaCl suggest that amino acids are very likely to be absorbed in humans. Mixture with a hypertonic level of NaCl exerts a cytotoxic effect in intestinal cells resulting in a loss of epithelium integrity. Results show that isotonic mixture of amino acids extracted from codfish salting wastewater could be used in food, feed, cosmetic and pharmaceutical formulations. These applications could contribute to the fish industry sustainability.