New insights into lipid and carbohydrate metabolism in teleost fish: transcriptional and functional characterization of adipocytes
The aim of the present thesis is directed towards the comprehension of the mechanisms involved in the regulation of lipid and carbohydrate metabolism in relevant species in European aquaculture. Special attention has been placed on adipose tissue due to its key role in the control of the overall ene...
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Universitat de Barcelona
2016
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ftubarcelona:oai:www.tdx.cat:10803/386387 2023-12-24T10:15:04+01:00 New insights into lipid and carbohydrate metabolism in teleost fish: transcriptional and functional characterization of adipocytes Bou Mira, Marta Navarro Álvarez, Isabel Universitat de Barcelona. Departament de Fisiologia i Immunologia 2016-02-04 application/pdf 178 p. http://hdl.handle.net/10803/386387 eng eng Universitat de Barcelona http://hdl.handle.net/10803/386387 ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs. info:eu-repo/semantics/openAccess TDX (Tesis Doctorals en Xarxa) Teleostis Teleósteos Teleostei Metabolisme Metabolismo Metabolism Teixit adipós Tejido adiposo Adipose tissues Ciències Experimentals i Matemàtiques 59 info:eu-repo/semantics/doctoralThesis info:eu-repo/semantics/publishedVersion 2016 ftubarcelona 2023-11-30T06:33:40Z The aim of the present thesis is directed towards the comprehension of the mechanisms involved in the regulation of lipid and carbohydrate metabolism in relevant species in European aquaculture. Special attention has been placed on adipose tissue due to its key role in the control of the overall energy homeostasis and its endocrine functions. Six diets were formulated to determine the feasibility of replacing dietary lipids (from 23 to 17%) by starch (from 12 to 28%) and subsequent replacements in starch by fibre up to levels of 18% in gilthead sea bream. The dietary treatments did not affect fish growth nor feed utilization efficiency and triggered only moderate changes in lipid and carbohydrate metabolism. Gilthead sea bream adipose tissue exhibited an activation of the lipogenic pathway and a transcriptional regulation of relevant transcription factors directed to modulate the preferential use of lipids or carbohydrates according to availability. Despite of a lack of hepatic regulation of gluconeogenesis, an efficient metabolic hepatic adaptation to dietary starch was found through the modulation of enzymes directed to increase glucose metabolization and balance fatty acid uptake and lipolysis. These metabolic adaptations encourage the formulation of diets with a relatively high content of starch or fibre. In addition, the capacity of Atlantic salmon adipocytes to synthesise fatty acids from glucose has been demonstrated for the first time. However, the capacity of the pathway is relatively low, which might be contributing to the previously reported glucose intolerance in salmonids. Atlantic salmon adipocytes mainly transformed glucose to glycerol and Krebs-cycle intermediates. The effect of glucose on the transcriptional regulation of enzymes involved in glycolysis, the pentose phosphate pathway and glyceroneogenesis, highlights the relevance of carbohydrate metabolism in fish adipocytes as well as the metabolic adaptation of these cells to ensure the availability of necessary factors and other substrates ... Doctoral or Postdoctoral Thesis Atlantic salmon Universitat de Barcelona: Theses and Dissertations Online (TDX) Krebs ENVELOPE(-61.467,-61.467,-64.633,-64.633) |
institution |
Open Polar |
collection |
Universitat de Barcelona: Theses and Dissertations Online (TDX) |
op_collection_id |
ftubarcelona |
language |
English |
topic |
Teleostis Teleósteos Teleostei Metabolisme Metabolismo Metabolism Teixit adipós Tejido adiposo Adipose tissues Ciències Experimentals i Matemàtiques 59 |
spellingShingle |
Teleostis Teleósteos Teleostei Metabolisme Metabolismo Metabolism Teixit adipós Tejido adiposo Adipose tissues Ciències Experimentals i Matemàtiques 59 Bou Mira, Marta New insights into lipid and carbohydrate metabolism in teleost fish: transcriptional and functional characterization of adipocytes |
topic_facet |
Teleostis Teleósteos Teleostei Metabolisme Metabolismo Metabolism Teixit adipós Tejido adiposo Adipose tissues Ciències Experimentals i Matemàtiques 59 |
description |
The aim of the present thesis is directed towards the comprehension of the mechanisms involved in the regulation of lipid and carbohydrate metabolism in relevant species in European aquaculture. Special attention has been placed on adipose tissue due to its key role in the control of the overall energy homeostasis and its endocrine functions. Six diets were formulated to determine the feasibility of replacing dietary lipids (from 23 to 17%) by starch (from 12 to 28%) and subsequent replacements in starch by fibre up to levels of 18% in gilthead sea bream. The dietary treatments did not affect fish growth nor feed utilization efficiency and triggered only moderate changes in lipid and carbohydrate metabolism. Gilthead sea bream adipose tissue exhibited an activation of the lipogenic pathway and a transcriptional regulation of relevant transcription factors directed to modulate the preferential use of lipids or carbohydrates according to availability. Despite of a lack of hepatic regulation of gluconeogenesis, an efficient metabolic hepatic adaptation to dietary starch was found through the modulation of enzymes directed to increase glucose metabolization and balance fatty acid uptake and lipolysis. These metabolic adaptations encourage the formulation of diets with a relatively high content of starch or fibre. In addition, the capacity of Atlantic salmon adipocytes to synthesise fatty acids from glucose has been demonstrated for the first time. However, the capacity of the pathway is relatively low, which might be contributing to the previously reported glucose intolerance in salmonids. Atlantic salmon adipocytes mainly transformed glucose to glycerol and Krebs-cycle intermediates. The effect of glucose on the transcriptional regulation of enzymes involved in glycolysis, the pentose phosphate pathway and glyceroneogenesis, highlights the relevance of carbohydrate metabolism in fish adipocytes as well as the metabolic adaptation of these cells to ensure the availability of necessary factors and other substrates ... |
author2 |
Navarro Álvarez, Isabel Universitat de Barcelona. Departament de Fisiologia i Immunologia |
format |
Doctoral or Postdoctoral Thesis |
author |
Bou Mira, Marta |
author_facet |
Bou Mira, Marta |
author_sort |
Bou Mira, Marta |
title |
New insights into lipid and carbohydrate metabolism in teleost fish: transcriptional and functional characterization of adipocytes |
title_short |
New insights into lipid and carbohydrate metabolism in teleost fish: transcriptional and functional characterization of adipocytes |
title_full |
New insights into lipid and carbohydrate metabolism in teleost fish: transcriptional and functional characterization of adipocytes |
title_fullStr |
New insights into lipid and carbohydrate metabolism in teleost fish: transcriptional and functional characterization of adipocytes |
title_full_unstemmed |
New insights into lipid and carbohydrate metabolism in teleost fish: transcriptional and functional characterization of adipocytes |
title_sort |
new insights into lipid and carbohydrate metabolism in teleost fish: transcriptional and functional characterization of adipocytes |
publisher |
Universitat de Barcelona |
publishDate |
2016 |
url |
http://hdl.handle.net/10803/386387 |
long_lat |
ENVELOPE(-61.467,-61.467,-64.633,-64.633) |
geographic |
Krebs |
geographic_facet |
Krebs |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
TDX (Tesis Doctorals en Xarxa) |
op_relation |
http://hdl.handle.net/10803/386387 |
op_rights |
ADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs. info:eu-repo/semantics/openAccess |
_version_ |
1786201719626006528 |