Efficacy of a commercial disinfectant against Vibrio ordalii, Vibrio anguillarum, Francisella sp. and Infectious Pancreatic Necrosis Virus (IPNV) pathogens of Atlantic salmon (Salmo salar) farmed in Chile

• Published in: Archivos de medicina veterinaria
• Main Authors: Muller, M., Ilardi, P., Avendaño-Herrera, R.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Archivos de Medicina Veterinaria 2011
• Subjects: desinfectante; patógenos de peces; salmón del atlántico; desinfectant; Chilean fish pathogens; Atlantic salmon; Salmo salar
• Online Access: http://revistas.uach.cl/index.php/amv/article/view/5057; https://doi.org/10.4067/S0301-732X2011000100010

The efficacy of the disinfectant Duplalim®, a synergistic blend of superquats and glutaraldehyde, was analysedin vitroagainst 4 fish pathogens. All concentrations tested (1:200 to 1:400 dilutions) were efficacious on killingVibrio ordaliiandVibrio anguillarumin seawater after 30 s, being the level of reduction equal to 1.8 x 106CFU/ml. Higher concentration of Duplalim®(1:50 dilutions) and time of exposure (at least 5 min) is needed to kill completelyFrancisellasp, an intracellular freshwater pathogen. When Infectious Pancreatic Necrosis Virus (IPNV) was treated with 1:400 disinfectant dilution, this concentration had a significant effect after 2 minutes, regardless of the IPNV titres employed (concentration greater than 107.6TCID50/ml). Duplalim®was tested in large scale againstVibrionaceaemembers. In comparison to the controls (without the disinfectant), 1:400 dilutions of Duplalim®totally killedV. ordaliiandV. anguillarumin seawater as well as on the surface of the fishing net (used in the cages of cultured salmon) after 15 min. Cultivable bacteria remained constant in the buckets without the disinfectant (1.4 ± 0.3 × 106CFU/ml), regardless of the period sampled. In the case of the adherence on the fishing net, bacteria not exposed to the disinfectant were detected at a concentration of 7.6 ± 3.2 × 105CFU/ml. These data indicate that the use of Duplalim®againstV. ordalii,V. anguillarumand IPNV is effective in low concentration and short time of exposure (15 min at a concentration of 1:400 dilutions), while the intracellular pathogen requires higher concentration. En el presente trabajo se evaluó la eficaciain vitrodel desinfectante Duplalim®, una combinación sinérgica de glutaraldehído y sales de amonio cuaternario de cuarta generación, contra 4 patógenos de peces prevalentes de la salmonicultura chilena. Los resultados muestran que todas las concentraciones ensayadas (diluciones entre 1:200 a 1:400) fueron eficaces sobre los aislados deVibrio ordaliiyVibrio anguillarumpost-30 s de exposición, detectando ...