Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity

A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essenti...

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Published in:Scientific Reports
Main Authors: Wang, Xu Ping (author), Zhou, Peng Fei (author), Li, Zhi Gang (author), Yang, Bo (author), Hollmann, F. (author), Wang, Yong Hua (author)
Format: Article in Journal/Newspaper
Language:English
Published: 2017
Subjects:
Antarc*
Antarctica
Online Access:http://resolver.tudelft.nl/uuid:48239759-6d0c-4aea-a203-a1313270040c
https://doi.org/10.1038/srep44599
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spelling fttudelft:oai:tudelft.nl:uuid:48239759-6d0c-4aea-a203-a1313270040c 2024-04-28T08:02:28+00:00 Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity Wang, Xu Ping (author) Zhou, Peng Fei (author) Li, Zhi Gang (author) Yang, Bo (author) Hollmann, F. (author) Wang, Yong Hua (author) 2017-03-20 http://resolver.tudelft.nl/uuid:48239759-6d0c-4aea-a203-a1313270040c https://doi.org/10.1038/srep44599 en eng http://resolver.tudelft.nl/uuid:48239759-6d0c-4aea-a203-a1313270040c http://www.scopus.com/inward/record.url?scp=85016084909&partnerID=8YFLogxK Scientific Reports--2045-2322--b41041f7-88a3-432e-98a2-0bb69c328595 https://doi.org/10.1038/srep44599 © 2017 Xu Ping Wang, Peng Fei Zhou, Zhi Gang Li, Bo Yang, F. Hollmann, Yong Hua Wang journal article 2017 fttudelft https://doi.org/10.1038/srep44599 2024-04-09T23:43:17Z A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product. BT/Biocatalysis Article in Journal/Newspaper Antarc* Antarctica Delft University of Technology: Institutional Repository Scientific Reports 7 1
institution Open Polar
collection Delft University of Technology: Institutional Repository
op_collection_id fttudelft
language English
description A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product. BT/Biocatalysis
format Article in Journal/Newspaper
author Wang, Xu Ping (author)
Zhou, Peng Fei (author)
Li, Zhi Gang (author)
Yang, Bo (author)
Hollmann, F. (author)
Wang, Yong Hua (author)
spellingShingle Wang, Xu Ping (author)
Zhou, Peng Fei (author)
Li, Zhi Gang (author)
Yang, Bo (author)
Hollmann, F. (author)
Wang, Yong Hua (author)
Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
author_facet Wang, Xu Ping (author)
Zhou, Peng Fei (author)
Li, Zhi Gang (author)
Yang, Bo (author)
Hollmann, F. (author)
Wang, Yong Hua (author)
author_sort Wang, Xu Ping (author)
title Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
title_short Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
title_full Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
title_fullStr Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
title_full_unstemmed Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
title_sort engineering a lipase b from candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
publishDate 2017
url http://resolver.tudelft.nl/uuid:48239759-6d0c-4aea-a203-a1313270040c
https://doi.org/10.1038/srep44599
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_relation http://resolver.tudelft.nl/uuid:48239759-6d0c-4aea-a203-a1313270040c
http://www.scopus.com/inward/record.url?scp=85016084909&partnerID=8YFLogxK
Scientific Reports--2045-2322--b41041f7-88a3-432e-98a2-0bb69c328595
https://doi.org/10.1038/srep44599
op_rights © 2017 Xu Ping Wang, Peng Fei Zhou, Zhi Gang Li, Bo Yang, F. Hollmann, Yong Hua Wang
op_doi https://doi.org/10.1038/srep44599
container_title Scientific Reports
container_volume 7
container_issue 1
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