Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essenti...
Published in: | Scientific Reports |
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Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2017
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Subjects: | |
Online Access: | http://resolver.tudelft.nl/uuid:48239759-6d0c-4aea-a203-a1313270040c https://doi.org/10.1038/srep44599 |
Summary: | A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product. BT/Biocatalysis |
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