Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity

A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essenti...

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Bibliographic Details
Published in:Scientific Reports
Main Authors: Wang, Xu Ping (author), Zhou, Peng Fei (author), Li, Zhi Gang (author), Yang, Bo (author), Hollmann, F. (author), Wang, Yong Hua (author)
Format: Article in Journal/Newspaper
Language:English
Published: 2017
Subjects:
Antarc*
Antarctica
Online Access:http://resolver.tudelft.nl/uuid:48239759-6d0c-4aea-a203-a1313270040c
https://doi.org/10.1038/srep44599
Description
Summary:A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product. BT/Biocatalysis

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