Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation by using cryopreserved sperm samples

• Published in: Journal of Applied Ichthyology
• Main Authors: Müller, T., Matsubara, H., Kubara, Y., Horváth, Ákos, Asturiano Nemesio, Juan Francisco, Urbányi, B.
• Other Authors: Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal, Tokyo University of Agriculture and Technology, Mohamed bin Zayed Species Conservation Fund
• Format: Article in Journal/Newspaper
• Language: English
• Published: Blackwell Publishing 2017
• Subjects: hist; art; Nemesio; European eel
• Online Access: https://doi.org/10.1111/jai.13316; http://hdl.handle.net/10251/104754

S [EN] The aim of this study was to test the artificial seminal plasma (ASP) as the extender as well as methanol as the cryoprotectant for cryopreservation, and to collect information on the fertilizing capacity of cryopreserved sperm samples. Eggs from Japanese eel, Anguilla japonica, females were control-fertilized with native sperm diluted with ASP and cryopreserved sperm (ASP as extender [in 1:100 ratio] and 10% methanol in v/v final concentration). No statistical differences (p<.05) were among the measured parameters (hatching, survival after 10days post-hatch, malformation rates) between the two groups, thus this cryopreservation method can be used effectively for artificial propagation of the Japanese eel, Anguilla japonica. Tokyo University of Agriculture Strategic Research Program (TUA-SRP); Mohamed bin Zayed Species Conservation Fund, Grant/ Award Number: 162512761; Research Centre of Excellence, Grant/Award Number: 11476-3/ 2016/FEKUT Müller, T.; Matsubara, H.; Kubara, Y.; Horváth, Á.; Asturiano Nemesio, JF.; Urbányi, B. (2017). Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation by using cryopreserved sperm samples. Journal of Applied Ichthyology (Online). 33:550-552. doi:10.1111/jai.13316 Abe, T., Ijiri, S., Adachi, S., & Yamauchi, K. (2010). Development of an in vitro culture system for producing eel larvae from immature ovarian follicles in Japanese eel Anguilla japonica. Fisheries Science, 76(2), 257-265. doi:10.1007/s12562-010-0216-8 Asturiano, J., Sørensen, S., Pérez, L., Lauesen, P., & Tomkiewicz, J. (2016). First Production of Larvae Using Cryopreserved Sperm: Effects of Preservation Temperature and Cryopreservation on European Eel Sperm Fertilization Capacity. Reproduction in Domestic Animals, 51(4), 485-491. doi:10.1111/rda.12706 Kurokawa, T., Okamoto, T., Gen, K., Uji, S., Murashita, K., Unuma, T., … Tanaka, H. (2008). Influence of Water Temperature on Morphological Deformities in Cultured Larvae of Japanese Eel,Anguilla japonica, at Completion of Yolk ...