A high-throughput FTIR spectroscopy approach to assess adaptive variation in the chemical composition of pollen

Abstract The two factors defining male reproductive success in plants are pollen quantity and quality, but our knowledge about the importance of pollen quality is limited due to methodological constraints. Pollen quality in terms of chemical composition may be either genetically fixed for high perfo...

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Bibliographic Details
Published in:Ecology and Evolution
Main Authors: Mikael Ohlson, Siri Fjellheim, Murat Bağcıoğlu, Achim Kohler, Boris Zimmermann, Valeria Tafinstseva
Format: Article in Journal/Newspaper
Language:unknown
Published: Wiley 2017
Subjects:
Poa
Online Access:https://doi.org/10.1002/ece3.3619
http://hdl.handle.net/11250/2480562
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fece3.3619
https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.3619
https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ece3.3619
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5743575
http://europepmc.org/articles/PMC5743575
https://brage.bibsys.no/xmlui/handle/11250/2480562
https://academic.microsoft.com/#/detail/2767873902
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Summary:Abstract The two factors defining male reproductive success in plants are pollen quantity and quality, but our knowledge about the importance of pollen quality is limited due to methodological constraints. Pollen quality in terms of chemical composition may be either genetically fixed for high performance independent of environmental conditions, or it may be plastic to maximize reproductive output under different environmental conditions. In this study, we validated a new approach for studying the role of chemical composition of pollen in adaptation to local climate. The approach is based on high‐throughput Fourier infrared (FTIR) characterization and biochemical interpretation of pollen chemical composition in response to environmental conditions. The study covered three grass species, Poa alpina, Anthoxanthum odoratum, and Festuca ovina. For each species, plants were grown from seeds of three populations with wide geographic and climate variation. Each individual plant was divided into four genetically identical clones which were grown in different controlled environments (high and low levels of temperature and nutrients). In total, 389 samples were measured using a high‐throughput FTIR spectrometer. The biochemical fingerprints of pollen were species and population specific, and plastic in response to different environmental conditions. The response was most pronounced for temperature, influencing the levels of proteins, lipids, and carbohydrates in pollen of all species. Furthermore, there is considerable variation in plasticity of the chemical composition of pollen among species and populations. The use of high‐throughput FTIR spectroscopy provides fast, cheap, and simple assessment of the chemical composition of pollen. In combination with controlled‐condition growth experiments and multivariate analyses, FTIR spectroscopy opens up for studies of the adaptive role of pollen that until now has been difficult with available methodology. The approach can easily be extended to other species and environmental ...